Serum Response Factor SRF Antibody

Western Blot: Serum Response Factor SRF Antibody [NBP1-33063] - Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE, antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: Serum Response Factor SRF Antibody [NBP1-33063] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody [NBP1-33063] - Mouse lung SRF dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Western Blot: Serum Response Factor SRF Antibody [NBP1-33063] - Effects of CRA-CM on the expression of SRF, C-fos and CDK2 in SH-SY5Y cells. The expression levels of SRF, C-fos and CDK2 were measured by western blotting and cropped blots are displayed. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-15163-5), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody [NBP1-33063] - Mouse muscle at a dilution of 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Product Discontinued

Serum Response Factor SRF Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SRF. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (96%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Serum Response Factor SRF Antibody

• MCM1
• serum response factor (c-fos serum response element-binding transcriptionfactor)
• Serum Response Factor SRF
• serum response factor
• SRF

Background

This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Serum Response Factor SRF Antibody (NBP1-33063)(1)

Publication using NBP1-33063

• Park J, Lee J, Yeom Z et al. Neuroprotective effect of Ruminococcus albus on oxidatively stressed SH-SY5Y cells and animals Sci Rep. 2017-11-06 [PMID: 29109537] (WB, Human)

Bioinformatics

• Gene Symbol: SRF
• Entrez:
  • 6722(Human)
• Uniprot:
  • P11831(Human)