Serum Amyloid A1 Antibody (SAA15cc) - BSA Free

• Reactivity: Hu, Ca
• Applications: WB, ELISA
• Clone: SAA15cc
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Serum Amyloid A1 Antibody (SAA15cc) - BSA Free Summary

• Description: Novus Biologicals Mouse Serum Amyloid A1 Antibody (SAA15cc) - BSA Free (NB100-73077) is a monoclonal antibody validated for use in WB and ELISA. Anti-Serum Amyloid A1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Human SAA.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SAA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: We recommend to avoid using bovine serum albumin (BSA) as a buffer component or blocking agent for SAA immunoassay. In buffers, BSA can be replaced with 1% casein. When developing an SAA immunoassay in microtiter plates, it is important to prevent non-specific binding of SAA to the wells of a plate. Plates blocking procedure and antigen dilution buffer should be optimized to ensure that SAA non-specific binding to the plate wells is suppressed. Buffer containing 1% casein and 0.05% Tween 20 is suggested for recommended MAb combinations.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Serum Amyloid A1 Antibody (SAA15cc) - BSA Free

• MGC111216
• PIG4
• SAA
• SAA1
• SAA2
• serum amyloid A protein
• Serum Amyloid A1
• TP53I4
• tumor protein p53 inducible protein 4

Background

The serum amyloid A (SAA) family comprises a number of differentially expressed lipoproteins, acute phase SAA1and SAA2, the former being a major component in plasma, and constitutive SAA's (C-SAAs). Although the liver is the primary site of synthesis of both SAA types, extrhepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed it's importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations. SAA rises earlier and more sharply than CRP. SAA enhances the binding of HDL's to macrophages and thus helps the delivery of lipid to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease process. In addition, recent experiments suggest that SAA may play a "housekeeping" role in normal human tissues. Elevated levels of SAA over time predispose secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissues and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serum Amyloid A1 Antibody (NB100-73077)(1)

Publication using NB100-73077

• Amaral MS, Santos DW, Pereira ASA Et al. Rhesus macaques self-curing from a schistosome infection can display complete immunity to challenge Nature communications 2021-10-26 [PMID: 34702841] (LFA, Monkey)

Bioinformatics

• Gene Symbol: SAA1
• Entrez:
  • 6288(Human)
• Uniprot:
  • P02735()