Serum Amyloid A1 Antibody (902738) [Unconjugated]

Serum Amyloid A1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Amyloid A1 Monoclonal Antibody (Catalog # MAB30191) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 902738
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Serum Amyloid A1 Antibody (902738) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Serum Amyloid A1
  Accession # P0DJI8
• Specificity: Detects human Serum Amyloid A1 in direct ELISAs.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: SAA1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serum Amyloid A1 Antibody (902738) [Unconjugated]

• MGC111216
• PIG4
• SAA
• SAA1
• SAA2
• serum amyloid A protein
• Serum Amyloid A1
• TP53I4
• tumor protein p53 inducible protein 4

Background

Human serum amyloid A protein-1 (SAA-1) is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines.  It is secreted as a 12 kDa, 104 amino acid (aa), nonglycosylated polypeptide that displaces apoA1 in the HDL₃ complex (SwissProt #P02735).  The SAA-1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent.  The SAA-1 gene product differs from the SAA-2 gene product by only seven amino acids.  Circulating SAA-1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus.  Mature human SAA-1 is 72%, 82% and 72% aa identical to mature mouse, rabbit and hamster SAA-1, respectively.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serum Amyloid A1 Antibody (MAB30191)(3)

Publications using MAB30191

• Xiao-Wen Gan, Wang-Sheng Wang, Jiang-Wen Lu, Li-Jun Ling, Qiong Zhou, Hui-Juan Zhang et al. De novo Synthesis of SAA1 in the Placenta Participates in Parturition Frontiers in Immunology 2020-06-09 [PMID: 32582166] (Immunohistochemistry, Human)
• Xiao-Wen Gan, Wang-Sheng Wang, Jiang-Wen Lu, Li-Jun Ling, Qiong Zhou, Hui-Juan Zhang et al. De novo Synthesis of SAA1 in the Placenta Participates in Parturition Frontiers in Immunology 6/9/2020 [PMID: 32582166]
• MM Yeh, S Boukhar, B Roberts, N Dasgupta, SS Daoud Genomic variants link to hepatitis C racial disparities Oncotarget, 2017-08-01;8(35):59455-59475. 2017-08-01 [PMID: 28938650] (IHC, Human)

Bioinformatics

• Gene Symbol: SAA1
• Uniprot:
  • P0DJI8()