Serpin E2/PN1 Antibody (244404) [Alexa Fluor® 700]

• Reactivity: Mu
• Applications: WB, IP
• Clone: 244404
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 700

Serpin E2/PN1 Antibody (244404) [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Serpin E2/PN1
  Ser20-Pro397
  Accession # Q07235
• Specificity: Detects mouse Serpin E2/PN1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Serpin A1, recombinant mouse (rm) Serpin D1, or rmSerpin F2 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin E2/PN1 Antibody (244404) [Alexa Fluor® 700]

• GDN
• GdNPF
• GDNserine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 2
• glia-derived nexin
• glial-derived neurite promoting factor
• Peptidase inhibitor 7
• PI-7
• PI7DKFZp686A13110
• PN-1
• PN1nexin
• PNI
• Protease nexin 1
• Protease nexin I
• Proteinase Nexin 1
• Serpin E2
• serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 2

Background

Serpin E2, also known as protease nexin I or glial-derived nexin (GDN), is a member of the Serpin superfamily of the serine protease inhibitors (1). Serpin E2 is a potent inhibitor of thrombin, plasmin and plasminogen activators (2). It is differentially expressed during neuronal differentiation and is able to transform human embryonic kidney cells into neuron-like cells (3). Its over‑expression in mice leads to progressive neuronal and motor dysfunction in these animals (4). It is also over‑expressed in the majority of pancreatic carcinoma as well as gastric and colorectal cancer samples whereas it is weakly expressed in all normal pancreas and chronic pancreatitis tissue samples (5). It plays an important role in controlling male fertility because its knockout male mice show a marked impairment in fertility from the onset of sexual maturity and its abnormal expression is found in the semen of men with seminal dysfunction (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.