Human Serpin E1/PAI-1 ELISA Kit (Colorimetric)

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ELISA: Human Serpin E1/PAI-1 ELISA Kit (Colorimetric) [NBP1-83740] - Representative standard curve for human PAI-1 ELISA. Human PAI-1 was diluted in serial 2-fold steps in AssayBuffer (1x). Do not use this standard ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
78 - 5,000 pg/mL
Sensitivity
29.0 pg/mL

Order Details

Human Serpin E1/PAI-1 ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected.
Standard Curve Range
78 - 5,000 pg/mL
Sensitivity
29.0 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
5.0%
Intra-Assay
4.7%
Spike Recovery
The recovery ranged from 64.5% to 84.8% with an overall mean recovery of 72.2% for serum and from 44.8% to 68.4%with an overall mean recovery of 58.7% for plasma samples.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SERPINE1

Applications/Dilutions

Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human PAI-1
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Human PAI-1 Standard lyophilized, 10000 pg/mL upon reconstitution
  5. Biotin-Conjugate anti-human PAI-1 polyclonal antibody
  6. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  7. Streptavidin-HRP
  8. Substrate Solution (tetramethyl-benzidine)
  9. Stop Solution (1M Phosphoric acid)

Alternate Names for Human Serpin E1/PAI-1 ELISA Kit (Colorimetric)

  • Endothelial plasminogen activator inhibitor
  • Nexin
  • PAI1
  • PAI-1
  • PAI1PAI-1
  • PAISerpin E1
  • PLANH1
  • PLANH1plasminogen activator inhibitor 1
  • serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1
  • Serpin E1
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Background

Plasminogen activator inhibitor-1 (PAI-1) is the primary inhibitor of plasminogen activators in plasma, rapidly inactivating both tissue plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA). PAI-1 is a single-chain glycoprotein with a molecular weight of 47 kilodaltons. During fibrinolysis, tissue plasminogen activator (tPA) converts the inactive protein plasminogen into plasmin which plays a critical role in fibrinolysis by degrading fibrin and providing localized protease activity in a number of physiological functions. PAI-1 is synthesized in the liver and by endothelial cells, and its synthesis is regulated by several physiologic mediators, including endotoxin, interleukin-1, fibroblast growth factor-2, and lipids. Plasminogen activator inhibitor-1 is an important inhibitor of the fibrinolytic system, so elevated levels could suppress fibrinolysis and result in an increased risk of thrombosis. Increased PAI-1 levels have been shown to be associated with a number of atherosclerotic risk factors, PAI-1 has been shown to act as a prothrombic factor in both arterial and venous thromboembolic disorders. Increased levels of PAI-1 are associated with an increased incidence of acute coronary syndrome. PAI-1 levels are also increased in patients with chronic and acute coronary artery disease (CAD) and in patients who suffer restenosis after coronary angioplasty.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SERPINE1
Entrez