Serpin E1/PAI-1 Antibody

Western Blot: Serpin E1/PAI-1 Antibody [NB100-1498] - Staining of A549 (A), HepG2 (B) and HeLa (C) lysates (35 ug protein in RIPA buffer).Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence

Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NB100-1498] - Staining of paraffin embedded Human Placenta. Antibody at 4 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Western Blot: Serpin E1/PAI-1 Antibody [NB100-1498] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Serpin E1/PAI-1 Antibody Summary

• Description: Novus Biologicals Goat Serpin E1/PAI-1 Antibody (NB100-1498) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Serpin E1/PAI-1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-GFKIDDKGMAPALRH corresponding to internal region according to NP_000593.1, NP_001158885.1.
• Specificity: This antibody is expected to recognize both reported isoforms.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SERPINE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 - 6 ug/mL
  • Immunohistochemistry-Paraffin 4-6 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Serpin E1/PAI-1 Antibody

• Endothelial plasminogen activator inhibitor
• Nexin
• PAI1
• PAI-1
• PAI1PAI-1
• PAISerpin E1
• PLANH1
• PLANH1plasminogen activator inhibitor 1
• serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1
• Serpin E1
• serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Background

As a member of the serpin family of serine protease inhibitors, PAI1 inhibits both tissue type palsminogen activator (tPA) and urokinase type plasminogen activator (uPA). High PAI1 levels are associated with an increased risk of thromboembolic disease while PAI1 deficiency may represent an inherited autosomal recessive bleeding disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin E1/PAI-1 Antibody (NB100-1498)(2)

Publications using NB100-1498

• Wang X, Guo L, Huang J et al. Plasminogen Activator Inhibitor-1 Potentiates Neutrophil Infiltration and Tissue Injury in Colitis International Journal of Biological Sciences 2023-04-18 [PMID: 37151884] (ELISA)
• Baumruker T, Csonga R, Pursch E et al. Activation of mast cells by incorporation of cholesterol into rafts. Int Immunol 2003-10-01 [PMID: 13679390]

Bioinformatics

• Gene Symbol: SERPINE1
• Entrez:
  • 5054(Human)
• Uniprot:
  • NP_000593.1.(Human)