Serpin D1/Heparin Cofactor II Antibody

• Reactivity: Hu
• Applications: WB, IP, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Serpin D1/Heparin Cofactor II Antibody Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin D1/Heparin Cofactor II
  Gly20-Ser499
  Accession # P05546
• Specificity: Detects human Serpin D1/Heparin Cofactor II in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Serpin D1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SERPIND1
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Neutralization 6.6 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin D1/Heparin Cofactor II Antibody

• HC2
• HC2LS2
• HCF2
• HCF2clade D (heparin cofactor), member 1
• HC-II
• Heparin Cofactor II
• HLS2HCII
• leuserpin 2
• LS2
• Protease inhibitor leuserpin-2
• Serpin D1
• serpin peptidase inhibitor, clade D (heparin cofactor), member 1

Background

Serpin D1, also known as Heparin Cofactor II, is a member of the Serpin superfamily of the serine protease inhibitors (1). Similar to Serpins A5 and C1, it inhibits thrombin and this activity is enhanced by heparin. Interestingly, a C-terminal His-tagged recombinant Serpin D1 had enhanced heparin effect, which was maintained in a plasma-based thrombin inhibition assay (2). Congenital Serpin D1 deficiency is a potential risk factor for thrombosis (3). The full-length human cDNA was expressed and the purified protein corresponded to the secreted form with anti-thrombin activity.

1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
2. Bauman, S.J. et al. (1999) J. Biol. Chem. 274:34556.
3. Tollefsen, D.M. (2002) Arch. Pathol. Lab. Med. 126:1394.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SERPIND1
• Uniprot:
  • P05546()