Serpin D1/Heparin Cofactor II Antibody (25) - Azide and BSA Free

Western Blot: Serpin D1/Heparin Cofactor II Antibody (25) [NBP2-90429] - Anti-Serpin D1/Heparin Cofactor II rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 53 kDa

Immunoprecipitation: Serpin D1/Heparin Cofactor II Antibody (25) [NBP2-90429] - Mouse Serpin D1/Heparin Cofactor II was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 0.5 ul anti-Mouse Serpin D1/Heparin Cofactor II rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Serpin D1/Heparin Cofactor II rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa

• Reactivity: Mu
• Applications: WB, ELISA, IP
• Clone: 25
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Serpin D1/Heparin Cofactor II Antibody (25) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Serpin D1/Heparin Cofactor II (Accession#: NP_032249.3; Met 1-Ser 478).
• Specificity: No cross-reactivity in ELISA with: Human SerpinA1 A1AT Human SerpinA3 AACT Human SerpinA5 PROCI Mouse SerpinF2 AAP
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SERPIND1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000-1:50000
  • Immunoprecipitation 0.2-1 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Serpin D1/Heparin Cofactor II Antibody (25) - Azide and BSA Free

• HC2
• HC2LS2
• HCF2
• HCF2clade D (heparin cofactor), member 1
• HC-II
• Heparin Cofactor II
• HLS2HCII
• leuserpin 2
• LS2
• Protease inhibitor leuserpin-2
• Serpin D1
• serpin peptidase inhibitor, clade D (heparin cofactor), member 1

Background

The product encoded by this gene is a serine proteinase inhibitor which rapidly inhibits thrombin in the presence of dermatan sulfate or heparin. The gene contains five exons and four introns. This protein shares homology with antithrombin III and other members of the alpha 1-antitrypsin superfamily. Mutations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SERPIND1