Serpin C1/Antithrombin-III Antibody (OTI8D5) - Azide and BSA Free

Western Blot: Serpin C1/Antithrombin-III Antibody (OTI8D5) - Azide and BSA Free [NBP2-74113] - Analysis of extracts (10ug) from 10 Human tissue by using anti-SERPINC1 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Flow Cytometry: Serpin C1/Antithrombin-III Antibody (OTI8D5) - Azide and BSA Free [NBP2-74113] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Antithrombin III antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, CyTOF-ready
• Clone: OTI8D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Serpin C1/Antithrombin-III Antibody (OTI8D5) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human SERPINC1 (NP_000479) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SERPINC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Western Blot
• Theoretical MW: 52.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Serpin C1/Antithrombin-III Antibody (OTI8D5) - Azide and BSA Free

• Antithrombin-III
• AT3antithrombin-III
• ATIII
• ATIIIantithrombin III
• MGC22579
• serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
• serine-cysteine proteinase inhibitor clade C member 1
• Serpin C1
• serpin peptidase inhibitor, clade C (antithrombin), member 1

Background

Antithrombin III (ATH III) is a plasma protein synthesised in the liver. Normal plasma levels are 115 to 160mg/L. ATH III consists of a single polypeptide chain and has a molecular weight of 58kDa. ATH III is a serine protease inhibitor and regulates coagulation by the formation of covalently liked complexes. ATH III also has a heparin binding site and in the presence of heparin the inhibition of thrombin, factors IXa, Xa and XIa is enhanced 1000 fold. Low levels of ATH III (functional or concentration) are associated with deep vein thrombosis and pulmonary embolism. Antithrombin assays are based on immunologic, enzymatic, or clotting properties

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SERPINC1