Recombinant Human Serpin A8/Angiotensinogen His (C-Term) Protein

Bioactivity: Recombinant Human Serpin A8/Angiotensinogen His (C-Term) Protein [NBP3-18196] - Immobilized Human Serpin A8/Angiotensinogen at 1 ug/mL (100 uL/well) can bind Anti Serpin A8/Angiotensinogen rabbit Mab with a linear range of 0.78-2.44 ng/mL.

SDS-Page: Recombinant Human Serpin A8/Angiotensinogen His (C-Term) Protein [NBP3-18196] - Recombinant Human Serpin A8/Angiotensinogen Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 65-70 kDa.

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Concentration: LYOPH

Recombinant Human Serpin A8/Angiotensinogen His (C-Term) Protein Summary

• Description: A recombinant protein with a C-terminal 6xHis tag corresponding to the amino acids Asp34-Ala485 of Human Serpin A8/Angiotensinogen.

  Source: HEK293

  (Accession #: NP_000020.1)
  
  Store at -20C to -80C long term. After reconstitution, the product is stable at -20C for 3 months, and 2-8C for up to 1 week.

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized Human Serpin A8/Angiotensinogen at 1 ug/ml (100 ul/well) can bind Anti-Serpin A8/Angiotensinogen rabbit Mab with a linear range of 0.78-2.44 ng/ml.
• Source: HEK293
• Protein/Peptide Type: Recombinant Protein
• Gene: AGT
• Purity: >97%, by SDS-PAGE
• Endotoxin Note: < 0.1 EU/ug of the protein by LAL method

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4
• Concentration: LYOPH
• Purity: >97%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water

Alternate Names for Recombinant Human Serpin A8/Angiotensinogen His (C-Term) Protein

• AGT
• alpha-1 antiproteinase, antitrypsin
• Angiotensin II
• angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Angiotensinogen
• ANHU
• Aogen
• FLJ92595
• FLJ97926
• PAT
• pre-angiotensinogen
• serine (or cysteine) proteinase inhibitor
• Serpin A8
• SERPINA8angiotensin I

Background

Angiotensin II is encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: AGT