Mouse Serpin A8/Angiotensinogen ELISA Kit (Chemiluminescence)

ELISA: Mouse Serpin A8/Angiotensinogen ELISA Kit (Chemiluminescence) [NBP2-66667] - Samples were spiked with high concentrations of Mouse Serpin A8/Angiotensinogen and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Serpin A8/Angiotensinogen ELISA Kit (Chemiluminescence) [NBP2-66667] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 125.00-8000 pg/mL
• Sensitivity: 75.00 pg/mL

Mouse Serpin A8/Angiotensinogen ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Mouse AGT in samples. No significant cross-reactivity or interference between Mouse AGT and analogues was observed.
• Standard Curve Range: 125.00-8000 pg/mL
• Sensitivity: 75.00 pg/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 8.44%
• Intra-Assay: CV% < 9.62%
• Spike Recovery: 83-113%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: AGT

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Serpin A8/Angiotensinogen ELISA Kit (Chemiluminescence)

• AGT
• alpha-1 antiproteinase, antitrypsin
• Angiotensin II
• angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Angiotensinogen
• ANHU
• Aogen
• FLJ92595
• FLJ97926
• PAT
• pre-angiotensinogen
• serine (or cysteine) proteinase inhibitor
• Serpin A8
• SERPINA8angiotensin I

Background

Angiotensin II is encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Serpin A8/Angiotensinogen ELISA Kit (NBP2-66667). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: AGT