Serpin A8/Angiotensinogen Antibody (754438) [Alexa Fluor® 532]

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 754438
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 532

Serpin A8/Angiotensinogen Antibody (754438) [Alexa Fluor® 532] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Serpin A8/Angiotensinogen
  Asp25-Val477
  Accession # P11859
• Specificity: Detects mouse Serpin A8/Angiotensinogen in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Serpin A8/Angiotensinogen, rhSerpin A4, recombinant mouse (rm) Serpin A1, A3N, A11, B8, C1, E1, E2, I1, or rmPED
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A8/Angiotensinogen Antibody (754438) [Alexa Fluor® 532]

• AGT
• alpha-1 antiproteinase, antitrypsin
• Angiotensin II
• angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Angiotensinogen
• ANHU
• Aogen
• FLJ92595
• FLJ97926
• PAT
• pre-angiotensinogen
• serine (or cysteine) proteinase inhibitor
• Serpin A8
• SERPINA8angiotensin I

Background

Serpin A8 (serine proteinase inhibitor-clade A #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors.  It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I.  This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II.  Ang II induces vascoconstriction and aldosterone release.  Mature human Serpin A8 is 452 aa in length (aa 34-485).  It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41).  Serpin A8 may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg.  Over aa 34-485, human Serpin A8 shares 61% aa identity with mouse Serpin A8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.