Serpin A6/Cortisol Binding Globulin Antibody (930816) [Unconjugated]

Western blot shows lysates of rat olfactory bulb tissue, rat liver tissue, rat brain (cerebellum) tissue, and rat brain (hippocampus) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Rat Serpin A6 Monoclonal Antibody (Catalog # MAB3838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin A6 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Serpin A6 was detected in perfusion fixed frozen sections of rat brain (preoptic nucleus) using Mouse Anti-Rat Serpin A6 Monoclonal Antibody (Catalog # MAB3838) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of Rat Cerebullum, rat liver, rat olfactory bulb, loaded at 0.2 mg/mL. A specific band was detected for Serpin A6 at approximately 88 kDa (as indicated) using 10 µg/mL of Mouse Anti-Rat Serpin A6 Monoclonal Antibody (Catalog # MAB3838) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Product Discontinued

Serpin A6/Cortisol Binding Globulin Antibody (930816) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat Serpin A6
  Met1-Ala396
  Accession # P31211
• Specificity: Detects rat Serpin A6 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SERPINA6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A6/Cortisol Binding Globulin Antibody (930816) [Unconjugated]

• antitrypsin), member 6
• CBG
• CBGcorticosteroid-binding globulin
• corticosteroid binding globulin
• member 6
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• Serpin A6
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
• transcortin

Background

Serpin A6, also known as Corticosteroid-binding Globulin/CBG and Transcortin, is a 45‑60 kDa secreted glycoprotein member of the clade A serpin family of protease inhibitors. Although it is a serpin family member, it shows no protease inhibition. Serpin A6 is produced principally by hepatocytes, but is also expressed by hypothalamic neurons and anterior pituitary endocrine cells. Serpin A6 transports progesterone and > 90% of circulating cortisol, and likely has three functions: first it can be cleaved, releasing cortisol to the tissues; second, it regulates levels of free hormone available over time; and third, it likely binds to specific cell-surface receptors when complexed to cortisol. Mature rat Serpin A6 is 374 amino acids (aa) in length (aa 23‑396). It contains an RCL segment that is involved in steroid binding (aa 334‑351). Over aa 23‑396, rat Serpin A6 shares 78% and 60% aa sequence identity with mouse and human Serpin A6, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SERPINA6
• Uniprot:
  • P31211()