SERCA3 ATPase Antibody - BSA Free

Immunohistochemistry-Paraffin: SERCA3 ATPase Antibody [NBP2-20306] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Western Blot: SERCA3 ATPase Antibody [NBP2-20306] - Sample (30 ug of whole cell lysate) A: BCL-1 5% SDS PAGE gel, diluted at 1:5000.

Western Blot: SERCA3 ATPase Antibody [NBP2-20306] - Jurkat whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SERCA3 ATPase antibody [N1N2], N-term (NBP2-20306) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA3 antibody [N1N2], N-term (NBP2-20306) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Bv, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SERCA3 ATPase Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human SERCA3 ATPase. The exact sequence is proprietary.
• Localization: Nucleus membrane; Endoplasmic reticulum membrane; Sarcoplasmic reticulum membrane
• Predicted Species: Rat (99%), Xenopus (92%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP2A3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 114 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (92%), Chicken (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SERCA3 ATPase Antibody - BSA Free

• adenosine triphosphatase, calcium
• ATP2A3
• ATPase, Ca(2+)-transporting, ubiquitous
• ATPase, Ca++ transporting, ubiquitous
• Calcium pump 3
• EC 3.6.3
• EC 3.6.3.8
• sarco/endoplasmic reticulum Ca2+ ATPase
• sarco/endoplasmic reticulum Ca2+ -ATPase
• sarcoplasmic/endoplasmic reticulum calcium ATPase 3
• SERCA3 ATPase
• SERCA3
• SERCA3calcium-translocating P-type ATPase
• SR Ca(2+)-ATPase 3

Background

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ATP2A3
• Uniprot:
  • Q93084(Human)