PMCA4 Antibody (JA9)

Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.

Immunohistochemistry-Paraffin: PMCA4 Antibody (JA9) [NB300-569] - Normal biopsies of deparaffinized Human brain tissue.

Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Figure 1 illustrates immunolocalization of PMCA4 and CaSRO in human corneal epithelium. (A) Immunolocalization of PMCA4 (green) using NB300-569 on cell membranes of all cells in each layer of hCE. (B) Immunolocalization of CaSRO (red)on cell membranes of hCE. (C) Merged image. Figure 2 illustrates immunolocalization of PMCA4 and CaSRO in bovine corneal epithelium (bCE). (A) Immunolocalization of PMCA4 (green) on cell membranes of all cells in each layer of bCE. (B) Immunolocalization of CaSRO (red) on cell membranes of bCE mostly in wing- and squamous cell layers. (C) Merged image.

Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.

• Reactivity: Hu, Mu, Rt, Po, Am, Bv, Rb
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KD
• Clone: JA9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PMCA4 Antibody (JA9) Summary

• Immunogen: Purified human erythrocyte PMCA.
• Specificity: This is specific to the PMCA4 isoform.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP2B4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • ELISA 1:15000
  • Flow Cytometry 2 ug / 10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Frozen 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Knockdown Validated
• Application Notes: WB: Detects an approx. 133 and 129 kDA protein representing PMCA4a and PMCA4b ATPase respectively. May be useful in Flow.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23845592). Rabbit reactivity reported in scientific literature (PMID: 21139678). Bovine reactivity reported in scientific literature (PMID: 23715721). Amphibian reactivity reported in scientific literature (PMID: 15236230). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PMCA4 Antibody (JA9)

• ATP2B2plasma membrane calcium ATPase
• ATPase, Ca++ transporting, plasma membrane 4
• DKFZp686G08106
• DKFZp686M088
• EC 3.6.3
• EC 3.6.3.8
• Matrix-remodeling-associated protein 1
• matrix-remodelling associated 1
• MXRA1
• Plasma membrane calcium ATPase isoform 4
• Plasma membrane calcium pump isoform 4
• plasma membrane calcium pump
• plasma membrane calcium-transporting ATPase 4
• PMCA4b
• PMCA4PMCA4x
• sarcolemmal calcium pump

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PMCA4 Antibody (NB300-569)(8)

Publications using NB300-569

• Yeh YC, Lin YP, Kramer H, Parekh AB Single nucleotide polymorphisms in Orai1 associated with atopic dermatitis inhibit protein turnover, decrease calcium entry and disrupt calcium-dependent gene expression Hum. Mol. Genet. 2019 Oct 10 [PMID: 31600783] (WB, IP, KD, Human)
• Grabek A, Dolfi B, Klein B et al. Dual Sensing of Physiologic pH and Calcium by EFCAB9 Regulates Sperm Motility Cell May 30 2019 [PMID: 31056283] (WB, Human)
• Carvajal-Maldonado D, Byrum AK, Jackson J et al. Perturbing cohesin dynamics drives MRE11 nuclease-dependent replication fork slowing Nucleic Acids Res. Jun 18 2018 [PMID: 29917110] (WB, Human)
• Ma CT, Chyau CC, Hsu CC et al. Pepino polyphenolic extract improved oxidative, inflammatory and glycative stress in the sciatic nerves of diabetic mice. Food Funct. 2016 Jan 21 [PMID: 26791916] (WB, Mouse)
• Chung JJ, Shim SH, Everley RA et al. Structurally distinct ca(2+) signaling domains of sperm flagella orchestrate tyrosine phosphorylation and motility. Cell. 2014 May 08 [PMID: 24813608] (ICC/IF, Mouse)
• Chen JH, Tsai CW, Wang CP, Lin HH. Anti-atherosclerotic potential of gossypetin via inhibiting LDL oxidation and foam cell formation. Toxicol Appl Pharmacol 2013 Jul 8 [PMID: 23845592] (WB, Mouse)
• Chen JH, Wang CJ, Wang CP et al. Hibiscus sabdariffa leaf polyphenolic extract inhibits LDL oxidation and foam cell formation involving up-regulation of LXRalpha/ABCA1 pathway. Food Chem. 2013 Nov 1 [PMID: 23768373] (WB, Mouse)
• Ritchie MF, Samakai E, Soboloff J. STIM1 is required for attenuation of PMCA-mediated Ca(2+) clearance during T-cell activation. The EMBO Journal. 2012 Jan 13. [PMID: 22246182]

Review for PMCA4 Antibody (NB300-569) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 11/23/2011

Summary

• Application: Western Blot
• Sample Tested: Human
• Species: Human
• Comments: Works good!

Blocking

• Blocking Details: Blocking Time: 1 hour

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Secondary Antibody

• Secondary Description: Secondary Ab: mouse

Details

• Detection Notes: Detection Method: HRP

Comments

• Comments: Works good!

Bioinformatics

• Gene Symbol: ATP2B4
• Entrez:
  • 493(Human)
  • 29600(Rat)
• Uniprot:
  • P23634(Human)