PMCA4 Antibody (JA9)

Images

 
Immunocytochemistry/ Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.
Immunohistochemistry-Paraffin: PMCA4 Antibody (JA9) [NB300-569] - Normal biopsies of deparaffinized Human brain tissue.
Immunocytochemistry/ Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Figure 1 illustrates immunolocalization of PMCA4 and CaSRO in human corneal epithelium. (A) Immunolocalization of PMCA4 (green) using NB300-569 ...read more
Immunocytochemistry/ Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Am, Bv, RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KD
Clone
JA9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PMCA4 Antibody (JA9) Summary

Immunogen
Purified human erythrocyte PMCA.
Specificity
This is specific to the PMCA4 isoform.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ATP2B4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:15000
  • Flow Cytometry 2 ug / 10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Frozen 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Knockdown Validated
Application Notes
WB: Detects an approx. 133 and 129 kDA protein representing PMCA4a and PMCA4b ATPase respectively. May be useful in Flow.
Reviewed Applications
Read 1 Review rated 4
using
NB300-569 in the following applications:

Publications
Read Publications using
NB300-569 in the following applications:

  • 1 publication
  • IP
    1 publication
  • KD
    1 publication
  • WB
    6 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23845592). Rabbit reactivity reported in scientific literature (PMID: 21139678). Bovine reactivity reported in scientific literature (PMID: 23715721). Amphibian reactivity reported in scientific literature (PMID: 15236230). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PMCA4 Antibody (JA9)

  • ATP2B2plasma membrane calcium ATPase
  • ATPase, Ca++ transporting, plasma membrane 4
  • DKFZp686G08106
  • DKFZp686M088
  • EC 3.6.3
  • EC 3.6.3.8
  • Matrix-remodeling-associated protein 1
  • matrix-remodelling associated 1
  • MXRA1
  • Plasma membrane calcium ATPase isoform 4
  • Plasma membrane calcium pump isoform 4
  • plasma membrane calcium pump
  • plasma membrane calcium-transporting ATPase 4
  • PMCA4b
  • PMCA4PMCA4x
  • sarcolemmal calcium pump

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PMCA4 Antibody (NB300-569)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IP, KD, WB.


Filter By Application
ICC/IF
(1)
IP
(1)
KD
(1)
WB
(6)
All Applications
Filter By Species
Human
(3)
Mouse
(4)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB300-569 Applications Species
Yeh YC, Lin YP, Kramer H, Parekh AB Single nucleotide polymorphisms in Orai1 associated with atopic dermatitis inhibit protein turnover, decrease calcium entry and disrupt calcium-dependent gene expression Hum. Mol. Genet. 2019 Oct 10 [PMID: 31600783] (WB, IP, KD, Human) WB, IP, KD Human
Grabek A, Dolfi B, Klein B et al. Dual Sensing of Physiologic pH and Calcium by EFCAB9 Regulates Sperm Motility Cell May 30 2019 [PMID: 31056283] (WB, Human) WB Human
Carvajal-Maldonado D, Byrum AK, Jackson J et al. Perturbing cohesin dynamics drives MRE11 nuclease-dependent replication fork slowing Nucleic Acids Res. Jun 18 2018 [PMID: 29917110] (WB, Human) WB Human
Ma CT, Chyau CC, Hsu CC et al. Pepino polyphenolic extract improved oxidative, inflammatory and glycative stress in the sciatic nerves of diabetic mice. Food Funct. 2016 Jan 21 [PMID: 26791916] (WB, Mouse) WB Mouse
Chung JJ, Shim SH, Everley RA et al. Structurally distinct ca(2+) signaling domains of sperm flagella orchestrate tyrosine phosphorylation and motility. Cell. 2014 May 08 [PMID: 24813608] (ICC/IF, Mouse) ICC/IF Mouse
Chen JH, Tsai CW, Wang CP, Lin HH. Anti-atherosclerotic potential of gossypetin via inhibiting LDL oxidation and foam cell formation. Toxicol Appl Pharmacol 2013 Jul 8 [PMID: 23845592] (WB, Mouse) WB Mouse
Chen JH, Wang CJ, Wang CP et al. Hibiscus sabdariffa leaf polyphenolic extract inhibits LDL oxidation and foam cell formation involving up-regulation of LXRalpha/ABCA1 pathway. Food Chem. 2013 Nov 1 [PMID: 23768373] (WB, Mouse) WB Mouse
Ritchie MF, Samakai E, Soboloff J. STIM1 is required for attenuation of PMCA-mediated Ca(2+) clearance during T-cell activation. The EMBO Journal. 2012 Jan 13. [PMID: 22246182]

Review for PMCA4 Antibody (NB300-569) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB300-569:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
WB Human 11/23/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsWorks good!

Blocking

Blocking DetailsBlocking Time: 1 hour

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: mouse

Details

Detection NotesDetection Method: HRP

Comments

CommentsWorks good!

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PMCA4 Antibody (NB300-569) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Recent Reviews

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1
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Anonymous
11/23/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol ATP2B4
Entrez
Uniprot