SERCA2 ATPase Antibody

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Western Blot: SERCA2 ATPase Antibody [NB100-237] - Detection of Human and Mouse SERCA2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using ...read more
Western Blot: SERCA2 ATPase Antibody [NB100-237] - Detection of Pig, Rat and Rabbit SERCA2 on HeLa whole cell lysate using NB100-237.
Immunoprecipitation: SERCA2 ATPase Antibody [NB100-237] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, RbSpecies Glossary
Applications WB, ChIP, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

SERCA2 ATPase Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 475 and 525 of human ATPase, Ca++ transporter, cardiac muscle, slow twitch 2 using the numbering given in entry NP_733765.1 (GeneID 488).
Localization
Endoplasmic reticulum + Sarcoplasmic reticulum membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP2A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This antibody can be used for Western Blot, where a band is seen around 110 kDa. Use in Immunoprecipitation reported in scientific literature (PMID 24284412) Use in chromatin immunoprecipitation reported in scientific literature (PMID 24284412)
Positive Control
Heart Aorta Valve Lysate (NB820-59218)
Publications
Read Publications using
NB100-237 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with X. laevis, X. tropicalis, Chicken, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Duckbill platypus, Tasmanian devil and Northern white-cheeked gibbon. Mouse reactivity reported in scientific literature (PMID:23516438).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SERCA2 ATPase Antibody

  • ATP2BFLJ20293
  • ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2
  • ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
  • Calcium pump 2
  • Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletalmuscle isoform
  • cardiac Ca2+ ATPase
  • DAR
  • DD
  • EC 3.6.3
  • EC 3.6.3.8
  • Endoplasmic reticulum class 1/2 Ca(2+) ATPase
  • FLJ38063
  • MGC45367
  • sarcoplasmic/endoplasmic reticulum calcium ATPase 2
  • SERCA2DKFZp686P0211
  • SR Ca(2+)-ATPase 2

Background

Sarcoplasmic/endoplasmic reticulum Calcium-ATPase 2 (SERCA2) is a magnesium-dependent pump that catalyzes the hydrolysis of ATP and translocates calcium from the cytosol to the sarcoplasmic reticulum lumen. There are 2 SERCA2 isoforms that differ in the 3' UTR. The SERCA2A isoform is highly expressed in heart and slow twitch skeletal muscle and is important to the regulation of the contraction and relaxation cycle, while the SERCA2B isoform is expressed in smooth muscle and adult epidermis. SERCA2 is also known as ATP2A2, ATP2B, DAR, DD, and MGC45367.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SERCA2 ATPase Antibody (NB100-237)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ChIP, ICC/IF, IP, WB.


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ChIP
(1)
ICC/IF
(1)
IP
(1)
WB
(4)
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Human
(3)
Mouse
(1)
Rat
(1)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-237 Applications Species
Dunay GA, Paragi P, Sara L et al. Depressed calcium cycling contributes to lower ischemia tolerance in hearts of estrogen-deficient rats. Menopause. 2014 Dec 15 [PMID: 25513985] (WB, Rat) WB Rat
Nensa FM, Neumann MH, Schroetter A et al. FE65 interactomics revealed SV2A and SERCA2 as new binding proteins in the human brain. Mol Cell Proteomics. 2013 Nov 27 [PMID: 24284412] (WB, ChIP, IP, Human) WB, ChIP, IP Human
Barriga M, Cal R, Cabello N et al. Low Density Lipoproteins Promote Unstable Calcium Handling Accompanied by Reduced SERCA2 and Connexin-40 Expression in Cardiomyocytes PLoS One 2013 [PMID: 23516438] (WB, Mouse) WB Mouse
Wang Y, Bruce AT, Tu C et al. Protein aggregation of SERCA2 mutants associated with Darier disease elicits ER stress and apoptosis in keratinocytes. J Cell Sci. 2011 Nov 1 [PMID: 22045735] (ICC/IF, Human) ICC/IF Human
Rich S, Pogoriler J, Husain AN et al. Long-term effects of epoprostenol on the pulmonary vasculature in idiopathic pulmonary arterial hypertension. Chest 2010 Nov [PMID: 21051399]
Li Q, Hueckstaedt LK, Ren J et al. The protease inhibitor UCF-101 ameliorates streptozotocin-induced mouse cardiomyocyte contractile dysfunction in vitro: role of AMP-activated protein kinase. Exp Physiol 2009 Sep [PMID: 19561140]
Prasad AM, Inesi G. Effects of Thapsigargin Phenylephrine on Calcineurin Protein Kinase C Signaling Functions in Cardiac Myocytes. 2009:00594. 2008 [PMID: 19244478]
Song J, Zhang XQ, Wang J et al. Regulation of cardiac myocyte contractility by phospholemman: Na+/Ca2+ exchange versus Na+ -K+ -ATPase. Am J Physiol Heart Circ Physiol 2008 Oct [PMID: 18708446]
Leatherbury L, Yu Q, Chatterjee B et al. A novel mouse model of X-linked cardiac hypertrophy. Am J Physiol Heart Circ Physiol;294(6):H2701-2711. 2008 [PMID: 18424640]
Yang J, Pawlyk B, Wen XH et al. Mpp4 is required for proper localization of plasma membrane calcium ATPases and maintenance of calcium homeostasis at the rod photoreceptor synaptic terminals. Hum Mol Genet 2007 May [PMID: 17341488]
Show All 13 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

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Blogs on SERCA2 ATPase.

Mending a Broken Heart: New SERCA2 Gene Therapy Fights Heart Disease
While many of the proteins on our antibody database are studied in relation to their expression in diseases; others become therapies in their own right. This is the case with SERCA2 (Sarcoplasmic reticulum Calcium-ATPase 2 pump), which recently hit th...  Read full blog post.

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Bioinformatics

Gene Symbol ATP2A2
Entrez
Uniprot