Western Blot: SERCA2 ATPase Antibody [NB100-237] - Detection of Human and Mouse SERCA2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using ...read more
Western Blot: SERCA2 ATPase Antibody [NB100-237] - Detection of Pig, Rat and Rabbit SERCA2 on HeLa whole cell lysate using NB100-237.
Immunoprecipitation: SERCA2 ATPase Antibody [NB100-237] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified ...read more
The immunogen recognized by this antibody maps to a region between residue 475 and 525 of human ATPase, Ca++ transporter, cardiac muscle, slow twitch 2 using the numbering given in entry NP_733765.1 (GeneID 488).
Endoplasmic reticulum + Sarcoplasmic reticulum membrane protein
Immunogen affinity purified
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This antibody can be used for Western Blot, where a band is seen around 110 kDa. Use in Immunoprecipitation reported in scientific literature (PMID 24284412) Use in chromatin immunoprecipitation reported in scientific literature (PMID 24284412)
Based on 100% sequence identity, this antibody is predicted to react with X. laevis, X. tropicalis, Chicken, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Duckbill platypus, Tasmanian devil and Northern white-cheeked gibbon. Mouse reactivity reported in scientific literature (PMID:23516438).
Sarcoplasmic/endoplasmic reticulum Calcium-ATPase 2 (SERCA2) is a magnesium-dependent pump that catalyzes the hydrolysis of ATP and translocates calcium from the cytosol to the sarcoplasmic reticulum lumen. There are 2 SERCA2 isoforms that differ in the 3' UTR. The SERCA2A isoform is highly expressed in heart and slow twitch skeletal muscle and is important to the regulation of the contraction and relaxation cycle, while the SERCA2B isoform is expressed in smooth muscle and adult epidermis. SERCA2 is also known as ATP2A2, ATP2B, DAR, DD, and MGC45367.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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