SENP1 Antibody (JG37-79)

Western Blot: SENP1 Antibody (JG37-79) [NBP2-76867] - Western blot analysis of SENP1 on K562 cell using anti-SENP1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: SENP1 Antibody (JG37-79) [NBP2-76867] - ICC staining SENP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: SENP1 Antibody (JG37-79) [NBP2-76867] - Immunohistochemical analysis of paraffin-embedded human small Intestine tissue using anti-SENP1 antibody. Counter stained with hematoxylin.

Flow Cytometry: SENP1 Antibody (JG37-79) [NBP2-76867] - Flow cytometric analysis of Daudi cells with SENP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: SENP1 Antibody (JG37-79) [NBP2-76867] - ICC staining SENP1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: SENP1 Antibody (JG37-79) [NBP2-76867] - ICC staining SENP1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: SENP1 Antibody (JG37-79) [NBP2-76867] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SENP1 antibody. Counter stained with hematoxylin.

Product Discontinued

SENP1 Antibody (JG37-79) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human SENP1 aa 380-521 / 644. (SwissProt: Q9P0U3 Human)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SENP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SENP1 Antibody (JG37-79)

• EC 3.4.22
• EC 3.4.22.-
• SENP1
• Sentrin/SUMO-specific protease SENP1
• sentrin-specific protease 1
• SUMO1/sentrin specific peptidase 1
• SUMO1/sentrin specific protease 1
• SuPr-2

Background

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SENP1