Semaphorin 5A Antibody - BSA Free

Immunohistochemistry-Paraffin: Semaphorin 5A Antibody [NBP1-80767] - Staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Semaphorin 5A Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PWTKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNTQPCPESWSEWSDWSECEASGVQVRARQCILLFPMGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGE
• Predicted Species: Mouse (94%), Rat (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEMA5A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Semaphorin 5A Protein (NBP1-80767PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Semaphorin 5A Antibody - BSA Free

• FLJ12815
• sema domain, seven thrombospondin repeats (type 1 and type 1-like)
• Sema F
• SEMA5A
• SEMAF
• Semaphorin 5A
• semaphorin F
• semaphorin-5A
• Semaphorin-F
• semF
• transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A

Background

Members of the semaphorin protein family are involved in axonal guidance during neural development. Semaphorin 5a is thought to have a bifunctional role in axon guidance, exerting both attractive and inhibitory effects on developing axons associated with limbic function. The thrombospondin repeats of Semaphorin 5a physically interact with both heparan sulfate proteoglycans and chondroitin sulfate proteoglycans, inducing attractive and inhibitory guidance cues, respectively. The biological responses to Semaphorin 5a are induced through binding to its high-affinity receptor, plexin B3, triggering intracellular signaling through Met.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Semaphorin 5A Antibody (NBP1-80767)(2)

Publications using NBP1-80767

• Maldonado LAG, Nascimento CR, Rodrigues Fernandes NA et al. Influence of tumor cell-derived TGF-beta on macrophage phenotype and macrophage-mediated tumor cell invasion The international journal of biochemistry & cell biology 2022-12-01 [PMID: 36343916] (IHC-P, Human)
• Jangjoo Ghalat N, Shahpasand K, Javan M Cis-p-tau plays crucial role in lysolecithin-induced demyelination and subsequent axonopathy in mouse optic chiasm Experimental neurology 2023-01-01 [PMID: 36343678] (IHC-Fr, Mouse)
  
  Details:
  Dilution used in IHC-Fr 1:100

Bioinformatics

• Gene Symbol: SEMA5A
• Entrez:
  • 9037(Human)
• Uniprot:
  • Q13591(Human)