Mouse Semaphorin 4D/CD100 ELISA Kit (Colorimetric)

ELISA: Mouse Semaphorin 4D/CD100 ELISA Kit (Colorimetric) [NBP2-75126] - Samples were spiked with high concentrations of Mouse Semaphorin 4D/CD100 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Semaphorin 4D/CD100 ELISA Kit (Colorimetric) [NBP2-75126] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Mouse Semaphorin 4D/CD100 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse CD100 in samples. No significant cross-reactivity or interference between Mouse CD100 and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 5
• Intra-Assay: %CV < 5.53
• Spike Recovery: 86 - 101%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SEMA4D

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Semaphorin 4D/CD100 ELISA Kit (Colorimetric)

• A8
• BB18
• CD100 antigen
• CD100
• CD100M-sema-G
• COLL-4
• FLJ33485
• FLJ34282
• FLJ39737
• FLJ46484
• immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D
• MGC169138
• MGC169141
• M-sema-G
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D
• SEMA4D
• SEMAJ
• Semaphorin 4D
• semaphorin-4D

Background

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100, a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SEMA4D