Semaphorin 4A Antibody

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Western blot shows lysates of human mature dendritic cells. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Semaphorin 4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4694) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Semaphorin 4A Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4A
Gly32-His683
Accession # Q9H3S1
Specificity
Detects human Semaphorin 4A in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse Semaphorin 4A is observed, and less than 1% cross-reactivity with recombinant human (rh) Semaphorin 4B, rhSemaphorin 4C, and rhSemaphorin 4D is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
SEMA4A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 4A Antibody

  • CORD10
  • RP35
  • Sema B
  • sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4A
  • SEMA4A
  • SEMAB
  • Semaphorin 4A
  • semaphorin-4A
  • semaphorin-B
  • SEMB
  • SEMBFLJ12287

Background

Sema4A (Semaphorin 4A; also Sema B) is a class IV member of the semaphorin family of proteins. It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells. It reportedly binds to Tim-2 and Plexin D1 and B1. In the immune system, it serves to activate naïve T cells and induce a Th1 phenotype. In the nervous system, it induces growth cone collapse in neurons. Mature human Sema4A is an 80 kDa (predicted) type I transmembrane glycoprotein that is 729 amino acids (aa) in length (aa 33-761). It contains a 651 aa extracellular region (aa 33-683) that is characterized by the presence of one Sema domain (aa 36-494), a PSI region (aa 496-528), and an Ig-like C2-type domain (aa 573-631). The 57 aa cytoplasmic region contains two potential phosphorylation sites at Thr744 and Ser747. There are two potential splice variants. One contains a 37 aa substitution for aa 293-328, while a second contains an alternative start site at Met100 coupled to a deletion of aa 122-154. Sema4A is reportedly cleaved to generate both a monomeric and a  dimeric soluble isoforms. Over aa 32-683, human Sema4A shares 86% aa identity with mouse Sema4A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SEMA4A
Uniprot