Semaphorin 3E Antibody

Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NB100-55417] - Staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Semaphorin 3E Antibody Summary

• Immunogen: Peptide with sequence C-NGGRYGTTKDYPDD corresponding to internal region according to NP_036563.1, NP_001171600.1.
• Epitope: NGGRYGTTKDYPDD
• Specificity: This antibody is expected to recognise both reported isoforms (NP_036563.1; NP_001171600.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SEMA3E
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 1:200
  • Peptide ELISA Detection limit 1:128000
• Application Notes: WB: Preliminary experiments gave an approx. 35 kDa band in human prostate lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2 kDa band according to NP_036563.1. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human prostate shows membranous staining of secretory cells in the gland.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Semaphorin 3E Antibody

• (semaphorin) 3E
• coll-5
• KIAA0331M-SEMAH
• sema domain, immunoglobulin domain (Ig), short basic domain, secreted
• Sema3E
• SEMAH
• SEMAHM-SemaK
• Semaphorin 3E
• semaphorin-3E

Background

Semaphorins, such as SEMA3E, are characterized by a conserved domain of about 500 amino acids. These proteins are involved in embryonic development, and some behave as neural guidance molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Semaphorin 3E Antibody (NB100-55417)(1)

Publication using NB100-55417

• Gu C, Yoshida Y, Livet J et al. Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins. Science 2005-01-14 [PMID: 15550623]

Bioinformatics

• Gene Symbol: SEMA3E
• Entrez:
  • 9723(Human)
  • 20349(Mouse)
• Uniprot:
  • NP_036563.1(Human)