SDPR Antibody - Azide and BSA Free

Immunohistochemistry: SDPR Antibody [NBP1-44090] - Tissue: Spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Incubated with 5 ug/mL for 1 h at RT.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SDPR Antibody - Azide and BSA Free Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: SDPR Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of human SDPR. (Uniprot: O95810)
• Localization: Plasma membrane and caveolae of adipocytes
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAVIN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:60000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1 ug/ml
• Application Notes: This product is tested in ELISA and Immunostaining and useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~40.5 kDa corresponding to the appropriate cell lysate or extract.
  
  Use in IHC-Fr reported in scientific literature (PMID:34228647).

Reactivity Notes

Cross-reactivity with SDPR from other sources has not been determined

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for SDPR Antibody - Azide and BSA Free

• cavin-2
• Phosphatidylserine-binding protein
• PS-p68
• PS-p68serum deprivation response (phosphatidylserine-binding protein)
• SDPR
• SDR
• SDRphosphatidylserine binding protein
• serum deprivation response (phosphatidylserine binding protein)
• serum deprivation response
• serum deprivation-response protein

Background

PTRF (polymerase I and transcript release factor) was first identified as a TTF-I and RNA polymerase I (RNA Pol I) interacting protein that functions in the termination of RNA polymerase I transcription. PTRF has also been described as a protein that localizes to the plasma membrane and caveolae of adipocytes and whose localization is under the control of insulin. In this context, PTRF has been observed to associate with a lipase and have an extranuclear role in the regulation of lipolysis. PTRF is also known as FKSG13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SDPR Antibody (NBP1-44090)(3)

Publications using NBP1-44090

• Xie Y, He L, Lugano R Et Al. Key molecular alterations in endothelial cells in human glioblastoma uncovered through single-cell RNA sequencing JCI insight 2021-07-06 [PMID: 34228647] (IHC-Fr, IHC-P)
• Bastiani M, Liu L, Hill MM et al. MURC/Cavin-4 and cavin family members form tissue-specific caveolar complexes. J Cell Biol 2009-06-01 [PMID: 19546242]
• Hill MM, Bastiani M, Luetterforst R, Kirkham M, Kirkham A, Nixon SJ, Walser P, Abankwa D, Oorschot VM, Martin S, Hancock JF, Parton RG, PTRF-Cavin. a conserved cytoplasmic protein required for caveola formation and function. Cell 2008-01-11 [PMID: 18191225]

Bioinformatics

• Gene Symbol: CAVIN2
• Entrez:
  • 8436(Human)
• Uniprot:
  • BAG60757(Human)
  • O95810(Human)