SDHB Antibody (HL2251) - Azide and BSA Free

Western Blot: SDHB Antibody (HL2251) - Azide and BSA Free [NBP3-25504] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SDHB antibody [HL2251] (NBP3-25504) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: SDHB Antibody (HL2251) - Azide and BSA Free [NBP3-25504] - SDHB antibody [HL2251] detects SDHB protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. SDHB stained by SDHB antibody [HL2251] (NBP3-25504) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: SDHB Antibody (HL2251) - Azide and BSA Free [NBP3-25504] - SDHB antibody [HL2251] detects SDHB protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. SDHB stained by SDHB antibody [HL2251] (NBP3-25504) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: SDHB Antibody (HL2251) - Azide and BSA Free [NBP3-25504] - SDHB antibody [HL2251] detects SDHB protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded dog brain. SDHB stained by SDHB antibody [HL2251] (NBP3-25504) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, IHC
• Clone: HL2251
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SDHB Antibody (HL2251) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human SDHB.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SDHB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for SDHB Antibody (HL2251) - Azide and BSA Free

• EC 1.3.5.1
• FLJ92337
• IP
• Iron-sulfur subunit of complex II
• PGL4
• SDH
• SDH1
• SDH2
• SDHIP
• succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
• succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Background

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SDHB