SCL/Tal1 Antibody

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human SCL/Tal1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3360) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SCL/Tal1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SCL/Tal1 Antibody Summary

• Immunogen: E. coli-derived recombinant human SCL
  Arg199-Gly266
  Accession # P17542
• Specificity: Detects human SCL in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TAL1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCL/Tal1 Antibody

• BHLHA17
• bHLHa17tal-1
• Class A basic helix-loop-helix protein 17
• SCL
• SCLT-cell acute lymphocytic leukemia protein 1
• Stem Cell Protein
• Tal1
• TAL-1
• T-cell acute lymphocytic leukemia 1
• T-cell leukemia/lymphoma protein 5
• TCL5
• TCL5tal-1 product

Background

SCL, also known as TAL-1 (T-cell Acute Lymphocytic Leukemia-1 protein), is a 40 kDa basic helix-loop-helix transcription factor. It regulates hematopoiesis, angiogenesis and erythrocyte maturation. Activation of SCL by chromosomal translocation may be a cause of some T-cell acute lymphoblastic leukemia. Two alternative splice variants lacking the first 25 or 175 amino acid residues also exist. Human and mouse SCL share 93% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SCL/Tal1 Antibody (AF3360)(3)

Publications using AF3360

• Soltys J, Perrone C, Knight J, Mao-Draayer Y. PDGF-AA and BDNF Promote Neural Stem Cell Differentiation. Lab Chip 9/18/2020 [PMID: 32945325]
• Kaia Skaggs, Donna M. Martin, Bennett G. Novitch Regulation of spinal interneuron development by the Olig-related protein Bhlhb5 and Notch signaling Development 8/1/2011 [PMID: 21750031]
• Ishiguro K, Rice AM, Rice KP, Sartorelli AC Inhibition of all-trans retinoic acid-induced granulocytic differentiation of WEHI-3B D+ cells by forced expression of SCL (TAL1) and GATA-1. Leuk. Res., 2009-02-20;33(9):1249-54. 2009-02-20 [PMID: 19230972] (Western Blot, Human)

Bioinformatics

• Gene Symbol: TAL1
• Uniprot:
  • P17542()