SCF/c-kit Ligand Antibody [Biotin]

• Reactivity: Fe
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

SCF/c-kit Ligand Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant feline SCF/c‑kit Ligand
  Lys26-Ala190
  Accession # P79169
• Specificity: Detects feline SCF/c‑kit Ligand in Western blots. In Western blots, approximately 60% cross-reactivity with recombinant mouse SCF is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KITLG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCF/c-kit Ligand Antibody [Biotin]

• c-kit Ligand
• DCUA
• DFNA69
• DKFZp686F2250
• familial progressive hyperpigmentation 2
• FPH2
• FPHH
• KIT ligand
• Kitl
• KITLG
• KL-1
• Mast cell growth factor
• MGF
• MGFSHEP7
• SCF
• SCFStem cell factor
• SFc-Kit ligand
• SHEP7
• SLF
• steel factor

Background

Feline SCF (stem cell factor; also known as c-kit ligand) is a type I transmembrane (TM) glycoprotein that plays an important role in a number of fetal and adult developmental processes (1‑4). It is synthesized as a 274 amino acid (aa) precursor that contains a 25 aa signal sequence, a 190 aa extracellular region, a 23 aa TM segment and a 36 aa cytoplasmic tail (5). Within the extracellular region there are two intrachain disulfide bonds and four alpha -helices. Although the predicted molecular weight is 19 kDa, the native molecule is anywhere from 28‑40 kDa in size and reflects both N- and O-linked glycosylation (1). Glycosylation is not necessary for bioactivity (6). The transmembrane form of SCF can be cleaved proteolytically, generating a 165 aa soluble form. Circulating SCF exists as both a monomer and nondisulfide-linked homodimer, with monomer predominating (50% to 75%) (6). Both the soluble and TM forms have bioactivity. Their principal targets may be different, however (7). A second, alternate splice short form of feline SCF has been identified (5). It too, is membrane bound and contains 246 aa residues. It will not give rise to a soluble form, since alternate splicing removes the proteolytic cleavage site used in the long form. The ratio of long form to short form varies tissue to tissue (1). Soluble feline SCF shares 93%, 93%, 90%, 87%, and 78% aa sequence identity with porcine, canine, bovine, human and mouse SCF, respectively. Cells known to express SCF include endothelial cells, fibroblasts and keratinocytes (1).

1. Broudy, V.C. (1997) Blood 90:1345.
2. Nakagawa, S. and T. Kitoh (2000) Curr. Opin. Hematol. 7:133.
3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
4. Kang, J. and S.D. Der (2004) Curr. Opin. Immunol. 16:180.
5. Dunham, S.P. and D.E. Onions (1996) DNA Seq. 6:233.
6. Hsu, Y-R. et al. (1997) J. Biol. Chem. 272:6406.
7. Kapur, R. et al. (1998) Blood 91:879.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KITLG
• Uniprot:
  • P79169()