SCF/c-kit Ligand Antibody [Unconjugated]

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SCF/c‑kit Ligand was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2278) at 15 ...read more
Recombinant Canine SCF/c‑kit Ligand (Catalog # 2278-SC) stimulates pro­liferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant ...read more

Product Details

Summary
Reactivity CaSpecies Glossary
Applications WB, B/N, ELISA(Cap), ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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SCF/c-kit Ligand Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant canine SCF/c‑kit Ligand
Lys26-Ala190
Accession # Q06220
Specificity
Detects canine SCF/c‑kit Ligand in ELISAs and Western blots. In sandwich immunoassays, less than 20% cross-reactivity with recombinant feline SCF is observed, less than 8% cross-reactivity with recombinant human SCF is observed, and less than 0.5% cross-reactivity with recombinant mouse SCF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KITLG
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.1-0.3 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
Standard: Recombinant Canine SCF/c-kit Ligand (Catalog # 2278-SC)

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCF/c-kit Ligand Antibody [Unconjugated]

  • c-kit Ligand
  • DCUA
  • DFNA69
  • DKFZp686F2250
  • familial progressive hyperpigmentation 2
  • FPH2
  • FPHH
  • KIT ligand
  • Kitl
  • KITLG
  • KL-1
  • Mast cell growth factor
  • MGF
  • MGFSHEP7
  • SCF
  • SCFStem cell factor
  • SFc-Kit ligand
  • SHEP7
  • SLF
  • steel factor

Background

Canine SCF (stem cell factor; also known as c‑kit ligand) is a type I transmembrane (TM) glycoprotein that plays an important role in a number of fetal and adult developmental processes (1‑4). It is synthesized as a 274 amino acid (aa) precursor that contains a 25 aa signal sequence, a 190 aa extracellular region, a 23 aa TM segment and a 36 aa cytoplasmic tail (5). Within the extracellular region there are four potential N‑linked glycosylation sites, two intrachain disulfide bonds, and four alpha ‑helices. Although the predicted molecular weight is 19 kDa, the native molecule is anywhere from 28‑40 kDa in size and reflects both N‑ and O‑linked glycosylation (1). Glycosylation is not necessary for bioactivity (6). The transmembrane form of SCF can be cleaved proteolytically, generating a 165 aa soluble form. Circulating SCF exists as both a monomer and nondisulfide‑linked homodimer, with monomer predominating (50% to 75%) (6). Both the soluble and TM forms have bioactivity. Their principal targets may be different, however (7). A second, alternate splice short form of SCF has been identified in other species (1). It is membrane bound but lacks the proteolytic cleavage site found in the long form. Thus, it cannot give rise to a soluble molecule. No such isoform has been reported for canine, but it could be assumed to exist. The ratio of long form to short form varies from tissue to tissue (1). Soluble canine SCF shares 88%, 93%, 86%, 83%, 76%, 76%, 86% and 88% aa sequence identity with porcine, feline, bovine, human, mouse, rat, goat and equine SCF, respectively. Cells known to express SCF include endothelial cells, fibroblasts and keratinocytes (1).

  1. Broudy, V.C. (1997) Blood 90:1345.
  2. Nakagawa, S. and T. Kitoh (2000) Curr. Opin. Hematol. 7:133.
  3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
  4. Kang, J. and S.D. Der (2004) Curr. Opin. Immunol. 16:180.
  5. Dunham, S.P. and D.E. Onions (1996) DNA Seq. 6:233.
  6. Hsu, Y-R. et al. (1997) J. Biol. Chem. 272:6406.
  7. Kapur, R. et al. (1998) Blood 91:879.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Biotin BAF2278

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Bioinformatics

Gene Symbol KITLG
Uniprot