SCF/c-kit Ligand Antibody [Unconjugated]

255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Human SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA). The ND₅₀ is typically 0.05-0.3 µg/mL." > 255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Human SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA). The ND₅₀ is typically 0.05-0.3 µg/mL." title="Recombinant Human SCF/c-kit Ligand (255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Human SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA). The ND₅₀ is typically 0.05-0.3 µg/mL." />

Recombinant Human SCF/c-kit Ligand (<a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="SCF/c‑kit Ligand was detected in immersion fixed HTC-116 Human Colorectal Carcinoma cells (Positive control) and absent in U-937 Human Histiocytic Lymphoma cells (Negative control) using Goat Anti-Human SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

SCF/c‑kit Ligand was detected in immersion fixed HTC-116 Human Colorectal Carcinoma cells (Positive control) and absent in U-937 Human Histiocytic Lymphoma cells (Negative control) using Goat Anti-Human SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

255-SC) was serially diluted and captured by Mouse Anti-Human SCF/c‑kit Ligand Monoclonal Antibody (Catalog # MAB655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 255-SC) was serially diluted and captured by Mouse Anti-Human SCF/c‑kit Ligand Monoclonal Antibody (Catalog # MAB655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human SCF/c‑kit Ligand (Catalog # 255-SC) was serially diluted and captured by Mouse Anti-Human SCF/c‑kit Ligand Monoclonal Antibody (Catalog # MAB655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human SCF/c‑kit Ligand (Catalog # <a class=

• Reactivity: Hu
• Applications: WB, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SCF/c-kit Ligand Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SCF/c-kit Ligand
  Glu26-Ala189
  Accession # P21583
• Specificity: Detects human SCF/c-kit Ligand in direct ELISAs and Western blots. In direct ELISAs, less than 60% cross-reactivity with recombinant mouse SCF is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KITLG
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-25 ug/mL
  • Neutralization 0.05-0.3 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCF/c-kit Ligand Antibody [Unconjugated]

• c-kit Ligand
• DCUA
• DFNA69
• DKFZp686F2250
• familial progressive hyperpigmentation 2
• FPH2
• FPHH
• KIT ligand
• Kitl
• KITLG
• KL-1
• Mast cell growth factor
• MGF
• MGFSHEP7
• SCF
• SCFStem cell factor
• SFc-Kit ligand
• SHEP7
• SLF
• steel factor

Background

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice. An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform (corresponding to this recombinant protein), human SCF shares 75-83% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SCF/c-kit Ligand Antibody (AF-255-NA)(9)

Publications using AF-255-NA

• Catherine Overed-Sayer, Elena Miranda, Rebecca Dunmore, Elena Liarte Marin, Lorea Beloki, Doris Rassl et al. Inhibition of mast cells: a novel mechanism by which nintedanib may elicit anti-fibrotic effects Thorax 9/1/2020 [PMID: 32709610]
• Smith A, Bowers S, Stratman A, Davis G Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions. PLoS ONE, 2013-12-31;8(12):e85147. 2013-12-31 [PMID: 24391990] (Neutralization, Human)
• Choi W, Wolber R, Gerwat W The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin. J. Cell. Sci., 2010-08-24;123(0):3102-11. 2010-08-24 [PMID: 20736300] (Western Blot, Human)
• Huang B, Lei Z, Zhang GM, Li D, Song C, Li B, Liu Y, Yuan Y, Unkeless J, Xiong H, Feng ZH SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment. Blood, 2008-06-04;112(4):1269-79. 2008-06-04 [PMID: 18524989] (Western Blot, Human)
• Hsieh FH, Sharma P, Gibbons A, Goggans T, Erzurum SC, Haque SJ Human airway epithelial cell determinants of survival and functional phenotype for primary human mast cells. Proc. Natl. Acad. Sci. U.S.A., 2005-09-26;102(40):14380-5. 2005-09-26 [PMID: 16186496] (ICC, Human)
• Chandrashekran A, Gordon MY, Darling D, Farzaneh F, Casimir C Growth factor displayed on the surface of retroviral particles without manipulation of envelope proteins is biologically active and can enhance transduction. J Gene Med, 2004-11-01;6(11):1189-96. 2004-11-01 [PMID: 15468194] (ICC, Immunoprecipitation, Human)
• Wang J, Roy SK Growth differentiation factor-9 and stem cell factor promote primordial follicle formation in the hamster: modulation by follicle-stimulating hormone. Biol. Reprod., 2003-10-29;70(3):577-85. 2003-10-29 [PMID: 14585807] (Neutralization, Hamster)
• Briard D, Brouty-Boye D, Azzarone B, Jasmin C Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15. J. Immunol., 2002-05-01;168(9):4326-32. 2002-05-01 [PMID: 11970974] (Neutralization, Human)
• Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor. Blood, 2002-01-01;99(1):224-31. 2002-01-01 [PMID: 11756175] (Neutralization, Human)

Bioinformatics

• Gene Symbol: KITLG
• Uniprot:
  • P21583()