SCF/c-kit Ligand Antibody

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Recombinant Human SCF/c‑kit Ligand (Catalog # 255-SC) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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SCF/c-kit Ligand Antibody Summary

Immunogen
E. coli-derived recombinant human SCF/c-kit Ligand (R&D Systems, Catalog # 255-SC)
Glu26-Ala189
Accession # P21583
Specificity
Detects human SCF/c-kit Ligand in direct ELISAs and Western blots. In direct ELISAs, less than 60% cross-reactivity with recombinant mouse SCF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KITLG
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Neutralization 0.05-0.3 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF-255-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCF/c-kit Ligand Antibody

  • c-kit Ligand
  • DCUA
  • DFNA69
  • DKFZp686F2250
  • familial progressive hyperpigmentation 2
  • FPH2
  • FPHH
  • KIT ligand
  • Kitl
  • KITLG
  • KL-1
  • Mast cell growth factor
  • MGF
  • MGFSHEP7
  • SCF
  • SCFStem cell factor
  • SFc-Kit ligand
  • SHEP7
  • SLF
  • steel factor

Background

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice. An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform (corresponding to this recombinant protein), human SCF shares 75-83% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SCF/c-kit Ligand Antibody (AF-255-NA)(9)

We have publications tested in 2 confirmed species: Human, Hamster.

We have publications tested in 4 applications: ICC, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
ICC
(1)
Immunoprecipitation
(2)
Neutralization
(4)
Western Blot
(2)
All Applications
Filter By Species
Human
(8)
Hamster
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF-255-NA Applications Species
Smith A, Bowers S, Stratman A, Davis G Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions. PLoS ONE, 2013;8(12):e85147. 2013 [PMID: 24391990] (Neutralization, Human) Neutralization Human
Choi W, Wolber R, Gerwat W The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin. J. Cell. Sci., 2010;123(0):3102-11. 2010 [PMID: 20736300] (Western Blot, Human) Western Blot Human
Huang B, Lei Z, Zhang GM, Li D, Song C, Li B, Liu Y, Yuan Y, Unkeless J, Xiong H, Feng ZH SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment. Blood, 2008;112(4):1269-79. 2008 [PMID: 18524989] (Western Blot, Human) Western Blot Human
Hsieh FH, Sharma P, Gibbons A, Goggans T, Erzurum SC, Haque SJ Human airway epithelial cell determinants of survival and functional phenotype for primary human mast cells. Proc. Natl. Acad. Sci. U.S.A., 2005;102(40):14380-5. 2005 [PMID: 16186496] (ICC, Human) ICC Human
Chandrashekran A, Gordon MY, Darling D, Farzaneh F, Casimir C Growth factor displayed on the surface of retroviral particles without manipulation of envelope proteins is biologically active and can enhance transduction. J Gene Med, 2004;6(11):1189-96. 2004 [PMID: 15468194] (Immunoprecipitation, Human) Immunoprecipitation Human
Chandrashekran A, Gordon MY, Darling D, Farzaneh F, Casimir C Growth factor displayed on the surface of retroviral particles without manipulation of envelope proteins is biologically active and can enhance transduction. J Gene Med, 2004;6(11):1189-96. 2004 [PMID: 15468194] (Immunoprecipitation, Human) Immunoprecipitation Human
Wang J, Roy SK Growth differentiation factor-9 and stem cell factor promote primordial follicle formation in the hamster: modulation by follicle-stimulating hormone. Biol. Reprod., 2004;70(3):577-85. 2004 [PMID: 14585807] (Neutralization, Hamster) Neutralization Hamster
Briard D, Brouty-Boye D, Azzarone B, Jasmin C Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15. J. Immunol., 2002;168(9):4326-32. 2002 [PMID: 11970974] (Neutralization, Human) Neutralization Human
Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor. Blood, 2002;99(1):224-31. 2002 [PMID: 11756175] (Neutralization, Human) Neutralization Human

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Bioinformatics

Gene Symbol KITLG
Uniprot