SCC112 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: SCC112 Antibody [NBP1-87904] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.

Analysis using Anti-PDS5A antibody NBP1-87904 (A) shows similar pattern to independent antibody NBP2-38313 (B).

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Staining of human gastrointestinal, kidney, placenta and squamous epithelia using Anti-PDS5A antibody NBP1-87904 (A) shows similar protein distribution across tissues to independent antibody NBP2-38313 (B).

Staining of human kidney shows strong nuclear positivity in cells in tubules.

Staining of human tonsil shows strong nuclear positivity in squamous epithelial cells.

Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Staining of human rectum shows strong nuclear positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SCC112 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GRRPKSESQGNATKNDDLNKPINKGRKRAAVGQESPGGLEAGNAKAPKLQDLAKKAAPAERQIDLQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDS5A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SCC112 Protein (NBP1-87904PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SCC112 Antibody - BSA Free

• Cell proliferation-inducing gene 54 protein
• DKFZp686B19246
• FLJ41012
• KIAA0648MGC131948
• PDS5
• PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
• PIG54
• SCC-112MGC161503
• Sister chromatid cohesion protein 112
• sister chromatid cohesion protein PDS5 homolog A

Background

SCC-112 is a novel cell cycle-regulated molecule. It is a nuclear protein of ~147 kDa, suggested to be associated with normal cell growth and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SCC112 Antibody (NBP1-87904)(1)

Publication using NBP1-87904

• Su P, You L, Beerens C et al. Microscopy-based single-cell proteomic profiling reveals heterogeneity in DNA damage response dynamics Cell Reports Methods 2022-06-01 [PMID: 35784653] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: PDS5A