![Western Blot: SCC112 Antibody [NB100-426] - Samples : Nuclear extract (5 and 20 ug for WB, 1 mg for IP) from HeLa cells. Antibodies : Affinity purified rabbit anti-SCC-112 antibody used at 0.1 ug/ml for WB and at 2 ug/mg extract for IP. Immunoprecipitated SCC-112 was detected with affinity purified rabbit anti-SCC-112 antibody used at 0.06 ug/ml. Detection : Chemiluminescence with an exposure time of less than 5 minutes.](http://images.novusbio.com/fullsize/SCC112-Antibody-Western-Blot-NB100-426-img0010.jpg "Western Blot: SCC112 Antibody [NB100-426] - Samples : Nuclear extract (5 and 20 ug for WB, 1 mg for IP) from HeLa cells. Antibodies : Affinity purified rabbit anti-SCC-112 antibody used at 0.1 ug/ml for WB and at 2 ug/mg extract for IP. Immunoprecipitated SCC-112 was detected with affinity purified rabbit anti-SCC-112 antibody used at 0.06 ug/ml. Detection : Chemiluminescence with an exposure time of less than 5 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit SCC112 Antibody - BSA Free (NB100-426) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | A synthetic peptide mapping to a region between residues 1250 and 1297 (C-terminus) of human SCC-112 using the numbering given in entry NP_056015.1 (GeneID 23244). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PDS5A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western blot and immunoprecipitation. Immunohistochemistry has not been tested. *The investigator should determine the optimal working dilution for a specific application. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SCC112 Antibody (NB100-426)Find related products by research area.
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![Western Blot: SCC112 Antibody [NB100-426] - Detection of human SCC-112 in Western Blot and immunoprecipitation, using NB100-426. Samples: HeLa nuclear extracts. Exposure time of less than 5 minutes.](http://images.novusbio.com/fullsize/SCC112-Antibody-Western-Blot-NB100-426-img0001.jpg "Western Blot: SCC112 Antibody [NB100-426] - Detection of human SCC-112 in Western Blot and immunoprecipitation, using NB100-426. Samples: HeLa nuclear extracts. Exposure time of less than 5 minutes.")
![Western Blot: SCC112 Antibody [NB100-426] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SCC-112 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SCC112-Antibody-Western-Blot-NB100-426-img0009.jpg "Western Blot: SCC112 Antibody [NB100-426] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SCC-112 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry p63/TP73L Antibody [Unconjugated]](https://images.novusbio.com/images2/p63_AF1916_Immunohistochemistry_16919.jpg)
![Immunocytochemistry p63/TP73L Antibody [Unconjugated]](https://images.novusbio.com/images2/p63_AF1916_Immunocytochemistry__Immunofluorescence_16973.jpg)
![Immunocytochemistry/ Immunofluorescence p63/TP73L Antibody [Unconjugated]](https://images.novusbio.com/images/af1916_human-p63-tp73l-affinity-purified-polyclonal-ab-41202412401237.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")