Scaffold attachment factor B2 Antibody

Product Discontinued

Scaffold attachment factor B2 Antibody Summary

• Immunogen: Peptide with sequence C-GEHNQRLEEHQARA corresponding to internal region according to NP_055464.1.
• Epitope: internal region
• Specificity: This antibody is not expected to cross-react with SAFB1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SAFB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave bands at approx. 75 kDa band and 60 kDa band in lysates of HeLa cell lines after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 107 kDa band according to NP_055464.1. The 75 kDa band was successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Scaffold attachment factor B2 Antibody

• KIAA0138SAF-B2
• scaffold attachment factor B2

Background

Scaffold attachment factor B2 (SAFB2) is a chromatin associated protein that interacts with DNA elements termed S/MARs (scaffold or matrix attachment regions) but is not part of the nuclear matrix. SAFB2 is structurally and functionally similar to SAFB1 and appears to be a multifunctional protein involved in chromatin organization, mRNA processing and transcriptional regulation. SAFB2 has been demonstrated to interact with and function as a corepressor with many nuclear receptors such as estrogen receptor-alpha, PPAR-gamma, and FXR-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Scaffold attachment factor B2 Antibody (NBP1-36982)(1)

Publication using NBP1-36982

• Oesterreich S. Scaffold attachment factors SAFB1 and SAFB2: Innocent bystanders or critical players in breast tumorigenesis?players in breast tumorigenesis J Cell Biochem 2003-11-01 [PMID: 14587024]

Bioinformatics

• Gene Symbol: SAFB2
• Uniprot:
  • NP_055464.1(Human)