SC35 Antibody (SC-35)

Images

 
Western Blot: SC35 Antibody (SC-35) [NB100-1774] - SC-35 recombinant protein (3 ug) was separated by SDS-PAGE and probed with Mouse Anti-Splicing Factor SC-35 Clone: SC-35. The antibody was developed using Goat ...read more
Immunocytochemistry/ Immunofluorescence: SC35 Antibody (SC-35) [NB100-1774] - Gadd45a localization in nuclear speckles is RNase sensitive Immunofluorescence confocal microscopy of detergent-extracted RKO cells. Cells ...read more
Immunocytochemistry/ Immunofluorescence: SC35 Antibody (SC-35) [NB100-1774] - HS-68 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-Splicing ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Am, DrSpecies Glossary
Applications WB, ELISA, EM, ICC/IF, IHC-Fr, IHC-P, IP, IHC-FrFl, KO
Clone
SC-35
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SC35 Antibody (SC-35) Summary

Immunogen
Partially purified mammalian spliceosome.
Epitope
This antibody recognizes a phospho-epitope of the non-snRNP (small nuclear ribonucleoprotein particles) factor SC35.
Localization
Nuclear
Marker
Nuclear Speckle Marker
Specificity
Recognizes a phospho-epitope on the non-snRNP (small nuclear ribonucleoprotein particles) factor SC-35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SRSF2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Electron Microscopy 1:10 - 1:500
  • ELISA Indirect
  • Immunocytochemistry/Immunofluorescence 1:2000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Immunohistochemistry Free-Floating
  • Knockout Validated
Application Notes
Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 30015619). This SC35 Antibody (SC-35) is validated for IHC-Fr and IHC-Free Floating from a verified customer review. The antibody labels SC-35 as a speckled pattern in the nucleoplasm, excluding the nucleoli. Other uses include inhibition and depletion of splicing activity in nuclear extracts and immunoaffinity purification. IHC-P: See Nie et al 2002 paper where it appears no antigen retrieval is necessary. Block in normal serum 10% in TBS (pH 7.6) for 30min then incubate antibody overnight at 4C. Use ABC amplification- DAB detection. WB: Detects a doublet of approximately 35 kDa. *Optimal dilutions/concentrations should be determined by the end user.
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-1774 in the following applications:

Publications
Read Publications using
NB100-1774 in the following applications:

  • 1 publication
  • 1 publication
  • KO
    1 publication
  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31600344).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for SC35 Antibody (SC-35)

  • PR264
  • SC35
  • SC-35
  • serine/arginine-rich splicing factor 2
  • SFRS2
  • SFRS2A
  • splicing component, 35 kDa
  • splicing factor SC35
  • splicing factor, arginine/serine-rich 2
  • SR splicing factor 2
  • SRp30b

Background

SC35 is involved in pre-mRNA splicing and is found in the bodies in the nucleus referred to as speckles, SC 35 domains or splicing factor compartments (SFCs). The SC-35 clone is frequently used as a marker for these bodies, the role of which is controversial. Speckles correspond largely if not entirely to ultrastructures termed interchromatin granule clusters (IGCs). SC35 is excluded from the nucleolus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Am, Dr
Applications: WB, ELISA, EM, ICC/IF, IHC-Fr, IHC-P, IP, IHC-FrFl, KO

Publications for SC35 Antibody (NB100-1774)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC-P, KO, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
KO
(1)
WB
(1)
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Filter By Species
Human
(2)
Mouse
(1)
Rat
(2)
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Showing Publications 1 - 8 of 8.
Publications using NB100-1774 Applications Species
Lehman BJ, Lopez-Diaz FJ, Santisakultarm TP, et al. Dynamic regulation of CTCF stability and sub-nuclear localization in response to stress PLoS genetics Jan 1 2021 [PMID: 33411704] (WB, Human) WB Human
Wu N, Nguyen XN, Wang L et al. The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation PLoS Pathog. 2019 Oct 01 [PMID: 31600344] (KO, Human, Mouse) KO Human, Mouse
Malik AM, Miguez RA, Li X et al. Matrin 3-dependent neurotoxicity is modified by nucleic acid binding and nucleocytoplasmic localization Elife. 2018 Jul 16 [PMID: 30015619] (IHC-P, Rat) IHC-P Rat
Grindheim AK, Hollas H, Raddum AM et al. Reactive oxygen species exert opposite effects on Tyr23 phosphorylation of the nuclear and cortical pools of Annexin A2. J. Cell. Sci. 2015 Dec 07 [PMID: 26644180] (ICC/IF, Rat) ICC/IF Rat
Sytnikova YA, Kubarenko AV, Schafer A et al. Gadd45a is an RNA binding protein and is localized in nuclear speckles. PLoS One;6(1). 2011 Jan 7. [PMID: 21249130]
Nie GY et al. A potential molecular mechanism for regulating pre-mRNA splicing of implantation-related genes through unique uterine expression of splicing factor SC35 in women rhesus monkeys. Reproduction 124:209-17. 2002 [PMID: 12141933]
Fu XD Maniatis T. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature 343:437-41 (1990). [PMID: 2137203]
Petri et al. Dephosphorylation of survival motor neurons (SMN) by PPM1G/PP2C{gamma} gover. J. Cell Bio. 179:451. 2007 [PMID: 1833187]

Reviews for SC35 Antibody (NB100-1774) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Rat.

Reviews using NB100-1774:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Rat
(2)
All Species
Images Ratings Applications Species Date Details
 SC35 NB100-1774
Enlarge
5
reviewed by:
Soledad Barez
Rat 11/25/2020
View

Summary

Sample TestedBrain free floating slices,free-floating slices rat brain
SpeciesRat
Lot94053

Comments

CommentsCitrate Buffer Antigen Retrieval. 1:100 dilution, overnight incubation 4°.
Immunohistochemistry-Frozen SC35 NB100-1774
Enlarge
5
reviewed by:
Soledad Barez
IHC-Fr Rat 11/25/2020
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFresh frozen rat brain
SpeciesRat
Lot94053

Comments

Comments1:100 dilution, overnight incubation at 4°C.
No need for antigen retrieval.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SC35 Antibody (NB100-1774) (0)

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Secondary Antibodies

 

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Soledad Barez
11/25/2020
Application:
Species: Rat

Soledad Barez
11/25/2020
Application: IHC-Fr
Species: Rat

Bioinformatics

Gene Symbol SRSF2
Entrez