Western Blot: SC35 Antibody (SC-35) [NB100-1774] - SC-35 recombinant protein (3 ug) was separated on SDS-PAGE and probed with Mouse Anti-Splicing Factor SC-35 Clone: SC-35. The antibody was developed using Goat ...read more
Immunocytochemistry/ Immunofluorescence: SC35 Antibody (SC-35) [NB100-1774] - HS-68 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-Splicing ...read more
This antibody recognizes a phospho-epitope of the non-snRNP (small nuclear ribonucleoprotein particles) factor SC35.
Nuclear Speckle Marker
Recognizes a phospho-epitope on the non-snRNP (small nuclear ribonucleoprotein particles) factor SC-35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF.
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The antibody labels SC-35 as a speckled pattern in the nucleoplasm, excluding the nucleoli. Other uses include inhibition and depletion of splicing activity in nuclear extracts and immunoaffinity purification. IHC-P: See Nie et al 2002 paper where it appears no antigen retrieval is necessary. Block in normal serum 10% in TBS (pH 7.6) for 30min then incubate antibody overnight at 4C. Use ABC amplification- DAB detection. WB: Detects a doublet of approximately 35 kDa. *Optimal dilutions/concentrations should be determined by the end user.
Read Publications using NB100-1774 in the following applications:
SC35 is involved in pre-mRNA splicing and is found in the bodies in the nucleus referred to as speckles, SC 35 domains or splicing factor compartments (SFCs). The SC-35 clone is frequently used as a marker for these bodies, the role of which is controversial. Speckles correspond largely if not entirely to ultrastructures termed interchromatin granule clusters (IGCs). SC35 is excluded from the nucleolus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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