SC35 Antibody (SC-35)

Images

 
Western Blot: SC35 Antibody (SC-35) [NB100-1774] - SC-35 recombinant protein (3 ug) was separated on SDS-PAGE and probed with Mouse Anti-Splicing Factor SC-35 Clone: SC-35. The antibody was developed using Goat ...read more
Immunocytochemistry/ Immunofluorescence: SC35 Antibody (SC-35) [NB100-1774] - HS-68 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-Splicing ...read more

Product Details

Summary
Reactivity Hu, Rt, Am, DrSpecies Glossary
Applications WB, ELISA, EM, ICC/IF, IP
Clone
SC-35
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SC35 Antibody (SC-35) Summary

Immunogen
Partially purified mammalian spliceosome.
Epitope
This antibody recognizes a phospho-epitope of the non-snRNP (small nuclear ribonucleoprotein particles) factor SC35.
Localization
Nuclear
Marker
Nuclear Speckle Marker
Specificity
Recognizes a phospho-epitope on the non-snRNP (small nuclear ribonucleoprotein particles) factor SC-35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SRSF2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Electron Microscopy 1:10-1:500
  • ELISA Indirect
  • Immunocytochemistry/Immunofluorescence 1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
The antibody labels SC-35 as a speckled pattern in the nucleoplasm, excluding the nucleoli. Other uses include inhibition and depletion of splicing activity in nuclear extracts and immunoaffinity purification. IHC-P: See Nie et al 2002 paper where it appears no antigen retrieval is necessary. Block in normal serum 10% in TBS (pH 7.6) for 30min then incubate antibody overnight at 4C. Use ABC amplification- DAB detection. WB: Detects a doublet of approximately 35 kDa. *Optimal dilutions/concentrations should be determined by the end user.
Publications
Read Publications using
NB100-1774 in the following applications:

Reactivity Notes

Cross-reacts with Newt, Frog.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for SC35 Antibody (SC-35)

  • PR264
  • SC35
  • SC-35
  • serine/arginine-rich splicing factor 2
  • SFRS2
  • SFRS2A
  • splicing component, 35 kDa
  • splicing factor SC35
  • splicing factor, arginine/serine-rich 2
  • SR splicing factor 2
  • SRp30b

Background

SC35 is involved in pre-mRNA splicing and is found in the bodies in the nucleus referred to as speckles, SC 35 domains or splicing factor compartments (SFCs). The SC-35 clone is frequently used as a marker for these bodies, the role of which is controversial. Speckles correspond largely if not entirely to ultrastructures termed interchromatin granule clusters (IGCs). SC35 is excluded from the nucleolus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SC35 Antibody (NB100-1774)(5)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SRSF2
Entrez