SAV1 Antibody - Azide and BSA Free

Western Blot: SAV1 Antibody - Azide and BSA Free [SAV1] - Western blot analysis of lysates from wild type (WT) and SAV1 knockout (KO) HeLa(KO) cells, using SAV1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 50s.

Immunohistochemistry-Paraffin: SAV1 Antibody [NBP3-15584] - Immunohistochemistry of paraffin-embedded Human colon using [KO Validated] SAV1 Rabbit pAb (NBP3-15584) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: SAV1 Antibody [NBP3-15584] - Immunohistochemistry of paraffin-embedded Mouse kidney using [KO Validated] SAV1 Rabbit pAb (NBP3-15584) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: SAV1 Antibody [NBP3-15584] - Immunohistochemistry of paraffin-embedded Rat kidney using [KO Validated] SAV1 Rabbit pAb (NBP3-15584) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Western Blot: SAV1 Antibody [NBP3-15584] - Western blot analysis of extracts from normal (control) and [KO Validated] SAV1 knockout (KO) HeLa cells, using [KO Validated] SAV1 antibody (NBP3-15584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SAV1 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit SAV1 Antibody - Azide and BSA Free (NBP3-15584) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 199-383 of human SAV1 (NP_068590.1). LPLPPGWSVDWTMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVTYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQIVKMYEAYRQALLTELENRKQRQQWYAQQHGKNF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SAV1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for SAV1 Antibody - Azide and BSA Free

• 45 kDa WW domain protein
• hWW45
• protein salvador homolog 1
• salvador homolog 1 (Drosophila)
• SAV
• WW domain-containing
• WW45salvador
• WWP4,1700040G09Rik

Background

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a coiled-coil region. It is ubiquitously expressed in adult tissues. The encoded protein is 94% identical to the mouse protein at the amino acid level.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: SAV1