| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15584-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13282-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73998 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38635-20ul | |||
![Western Blot: SAV1 Antibody (3B2) [H00060485-M02] - MST2 and SAV1 increase the levels of PPAR-gamma by increasing its protein stability. Increasing amounts (0.5, 1, 3 ug) of Myc-SAV1 were co-expressed with Flag-PPAR gamma and/or HA-MST2 in 293 cells. At 48 h after transfection, cell lysates were immunoblotted with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0030983), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SAV1-Antibody-3B2-Western-Blot-H00060485-M02-img0012.jpg "Western Blot: SAV1 Antibody (3B2) [H00060485-M02] - MST2 and SAV1 increase the levels of PPAR-gamma by increasing its protein stability. Increasing amounts (0.5, 1, 3 ug) of Myc-SAV1 were co-expressed with Flag-PPAR gamma and/or HA-MST2 in 293 cells. At 48 h after transfection, cell lysates were immunoblotted with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0030983), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: SAV1 Antibody (3B2) [H00060485-M02] - Analysis of monoclonal antibody to SAV1 on HeLa cell. Antibody concentration 60 ug/ml.](http://images.novusbio.com/fullsize/SAV1-Antibody-3B2-Immunocytochemistry-Immunofluorescence-H00060485-M02-img0006.jpg)
![Western Blot: SAV1 Antibody (3B2) [H00060485-M02] - SAV1 monoclonal antibody (M02), clone 3B2. Analysis of SAV1 expression in HepG2.](http://images.novusbio.com/fullsize/SAV1-Antibody-3B2-Western-Blot-H00060485-M02-img0009.jpg)
![Immunoprecipitation: SAV1 Antibody (3B2) [H00060485-M02] - Analysis of SAV1 transfected lysate using anti-SAV1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SAV1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/SAV1-Antibody-3B2-Immunoprecipitation-H00060485-M02-img0007.jpg)
![ELISA: SAV1 Antibody (3B2) [H00060485-M02] - Detection limit for recombinant GST tagged SAV1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SAV1-Antibody-3B2-ELISA-H00060485-M02-img0011.jpg)
![Western Blot: SAV1 Antibody (3B2) [H00060485-M02] - MST2 & SAV1 increase the levels of PPAR gamma by increasing its protein stability.(A) Increasing amounts (0.5, 1, 3 µg) of Myc-SAV1 were co-expressed with Flag-PPAR gamma and/or HA-MST2 in 293 cells. At 48 h after transfection, cell lysates were immunoblotted with the indicated antibodies. (B) Flag-tagged PPAR gamma or PPAR gamma (128–505) was co-expressed with HA-MST2, HA-MST2-KD (inactive mutant), Myc-SAV1, and/or Myc-SAV1(1–201) in 293 cells as indicated. Cell lysates were prepared at various times after treatment with 40 µg/mL cycloheximide & then immunoblotted with an anti-Flag antibody. The PPAR gamma protein expression was quantified & the half-life of PPAR gamma protein was calculated. Expression of GAPDH was detected as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22292086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00060485-m02_mouse-monoclonal-sav1-antibody-3b2-310202415371988.jpg)
![Western Blot: SAV1 Antibody - Azide and BSA Free [SAV1] - Western blot analysis of lysates from wild type (WT) and SAV1 knockout (KO) HeLa(KO) cells, using SAV1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 50s.](http://images.novusbio.com/fullsize/nbp3-15584_rabbit-sav1-pab-5220251734423.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SAV1 Antibody - Azide and BSA Free [SAV1] - Western blot analysis of lysates from wild type (WT) and SAV1 knockout (KO) HeLa(KO) cells, using SAV1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 50s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: SAV1 Antibody [NBP3-15584] - Immunohistochemistry of paraffin-embedded Human colon using [KO Validated] SAV1 Rabbit pAb (NBP3-15584) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SAV1-Antibody-Immunohistochemistry-Paraffin-NBP3-15584-img0004.jpg)
![Immunohistochemistry-Paraffin: SAV1 Antibody [NBP3-15584] - Immunohistochemistry of paraffin-embedded Mouse kidney using [KO Validated] SAV1 Rabbit pAb (NBP3-15584) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SAV1-Antibody-Immunohistochemistry-Paraffin-NBP3-15584-img0002.jpg)
![Immunohistochemistry-Paraffin: SAV1 Antibody [NBP3-15584] - Immunohistochemistry of paraffin-embedded Rat kidney using [KO Validated] SAV1 Rabbit pAb (NBP3-15584) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SAV1-Antibody-Immunohistochemistry-Paraffin-NBP3-15584-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SAV1 Antibody [NBP3-15584] - Western blot analysis of extracts from normal (control) and [KO Validated] SAV1 knockout (KO) HeLa cells, using [KO Validated] SAV1 antibody (NBP3-15584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/SAV1-Antibody-Knockout-Validated-NBP3-15584-img0001.jpg)
![Western Blot: SAV1 Antibody [NBP2-13282] - Analysis in human cell lines U2OS and A-549 using anti-SAV1 antibody. Corresponding SAV1 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.](http://images.novusbio.com/fullsize/SAV1-Antibody-Western-Blot-NBP2-13282-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SAV1 Antibody [NBP2-13282] - Analysis in human cell lines U2OS and A-549 using anti-SAV1 antibody. Corresponding SAV1 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SAV1 Antibody [NBP2-13282] - Staining of human cell line SiHa shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SAV1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13282-img0006.jpg)
![Immunohistochemistry-Paraffin: SAV1 Antibody [NBP2-13282] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SAV1-Antibody-Immunohistochemistry-Paraffin-NBP2-13282-img0008.jpg)
![Western Blot: SAV1 Antibody [NBP2-13282] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/SAV1-Antibody-Western-Blot-NBP2-13282-img0010.jpg)
![Immunohistochemistry: SAV1 Antibody (PSH04-45) [NBP3-32931] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SAV1 antibody (NBP3-32931) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32931) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32931_rabbit-sav1-mab-psh04-45-298202414304199.jpg "Immunohistochemistry: SAV1 Antibody (PSH04-45) [NBP3-32931] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SAV1 antibody (NBP3-32931) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32931) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: SAV1 Antibody (PSH04-45) [NBP3-32931] - Flow cytometric analysis of HCT 116 cells labeling SAV1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32931, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32931_rabbit-sav1-mab-psh04-45-392024884625.jpg)
![Western Blot: SAV1 Antibody (PSH04-45) [NBP3-32931] - Western blot analysis of SAV1 on different lysates with Rabbit anti-SAV1 antibody (NBP3-32931) at 1/2,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: HEK-293 cell lysate (20 µg/Lane) Lane 5: MDA-MB-231 cell lysate (20 µg/Lane) Lane 6: SW480 cell lysate (20 µg/Lane) Lane 7: BxPC-3 cell lysate (20 µg/Lane) Lane 8: 786-0 cell lysate (negative) (20 µg/Lane) Lane 9: A549 cell lysate (20 µg/Lane) Lane 10: NIH/3T3 cell lysate (20 µg/Lane) Lane 11: PC-12 cell lysate (20 µg/Lane) Lane 12: Mouse testis tissue lysate (40 µg/Lane) Lane 13: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 3 minute; ECL: 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32931_rabbit-sav1-mab-psh04-45-3920248453522.jpg)
![Western Blot: SAV1 Antibody (2B7) [NBP2-45687] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAV1 .](http://images.novusbio.com/fullsize/SAV1-Antibody-2B7-Western-Blot-NBP2-45687-img0002.jpg "Western Blot: SAV1 Antibody (2B7) [NBP2-45687] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAV1 .")
![Immunohistochemistry: SAV1 Antibody (2B7) [NBP2-45687] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0) Dilution: 1:150](http://images.novusbio.com/fullsize/SAV1-Antibody-2B7-Immunohistochemistry-NBP2-45687-img0001.jpg)
![Western Blot: SAV1 Antibody (3A10) [H00060485-M04] - Analysis of SAV1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/SAV1-Antibody-3A10-Western-Blot-H00060485-M04-img0003.jpg "Western Blot: SAV1 Antibody (3A10) [H00060485-M04] - Analysis of SAV1 expression in Hela S3 NE (Cat # L013V3).")
![Sandwich ELISA: SAV1 Antibody (3A10) [H00060485-M04] - Detection limit for recombinant GST tagged SAV1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SAV1-Antibody-3A10-Sandwich-ELISA-H00060485-M04-img0002.jpg)
![Western Blot: SAV1 Antibody [NBP3-38635] - Western blot analysis of various lysates using SAV1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38635_rabbit-sav1-pab-912202415161853.jpg "Western Blot: SAV1 Antibody [NBP3-38635] - Western blot analysis of various lysates using SAV1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: SAV1 Overexpression Lysate (Adult Normal) [NBL1-15703] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SAV1.](http://images.novusbio.com/fullsize/SAV1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15703-img0002.jpg "Western Blot: SAV1 Overexpression Lysate (Adult Normal) [NBL1-15703] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SAV1.")