SAV1 Antibody - BSA Free

Western Blot: SAV1 Antibody [NBP2-13282] - Analysis in human cell lines U2OS and A-549 using anti-SAV1 antibody. Corresponding SAV1 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.

Immunocytochemistry/Immunofluorescence: SAV1 Antibody [NBP2-13282] - Staining of human cell line SiHa shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SAV1 Antibody [NBP2-13282] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Western Blot: SAV1 Antibody [NBP2-13282] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Staining of human cell line SiHa shows localization to cytosol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SAV1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: IGRVAATSLGNLTNHGSEDLPLPPGWSVDWTMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVTYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLA
• Predicted Species: Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SAV1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SAV1 Protein (NBP2-13282PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SAV1 Antibody - BSA Free

• 45 kDa WW domain protein
• hWW45
• protein salvador homolog 1
• salvador homolog 1 (Drosophila)
• SAV
• WW domain-containing
• WW45salvador
• WWP4,1700040G09Rik

Background

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a coiled-coil region. It is ubiquitously expressed in adult tissues. The encoded protein is 94% identical to the mouse protein at the amino acid level.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SAV1 Antibody (NBP2-13282)(1)

Publication using NBP2-13282

• Leach JP, Heallen T, Zhang M et al. Hippo pathway deficiency reverses systolic heart failure after infarction. Nature. 2017-10-12 [PMID: 28976966] (WB, Human)

Bioinformatics

• Gene Symbol: SAV1