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SATB1 Antibody (SP05-03)

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Western Blot: SATB1 Antibody (SP05-03) [NBP2-67371] - Analysis of SATB1 on human thymus lysates using anti-SATB1 antibody at 1/1,000 dilution.
Western Blot: SATB1 Antibody (SP05-03) [NBP2-67371] - Analysis of SATB1 on human thymus lysates using anti-SATB1 antibody at 1/1,000 dilution.
Immunohistochemistry-Paraffin: SATB1 Antibody (SP05-03) [NBP2-67371] - Analysis of paraffin-embedded rat spleen tissue using anti-SATB1 antibody. Counter stained with hematoxylin.
Flow Cytometry: SATB1 Antibody (SP05-03) [NBP2-67371] - Analysis of Jurkat cells with SATB1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). ...read more
Immunohistochemistry-Paraffin: SATB1 Antibody (SP05-03) [NBP2-67371] - Analysis of paraffin-embedded mouse thymus tissue using anti-SATB1 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
SP05-03
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SATB1 Antibody (SP05-03) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
C terminal Human SATB1
Localization
Nucleus matrix, Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SATB1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for SATB1 Antibody (SP05-03)

  • DNA-binding protein SATB1
  • SATB homeobox 1
  • SATB1
  • special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's)
  • Special AT-rich sequence-binding protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: CDP/CUTL1 Antibody (2A10) [H00001523-M01] - Analysis of CUX1 expression in transfected 293T cell line by CUTL1 monoclonal antibody (M01), clone 2A10.Lane 1: CUX1 transfected lysate(77.2 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: CDP/CUTL1 Antibody (2A10) [H00001523-M01] - Analysis of monoclonal antibody to CUTL1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: CDP/CUTL1 Antibody (2A10) [H00001523-M01] - Analysis of monoclonal antibody to CUTL1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 5 ug/ml.
(1 Review)
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Western Blot: IRF1 Antibody (SR44-08) [NBP2-67330] - Analysis of IRF1 on different lysates using anti-IRF1 antibody at 1/1,000 dilution. Positive control: Lane 1: PC-12 Lane 2: Jurkat
Immunocytochemistry/Immunofluorescence: IRF1 Antibody (SR44-08) [NBP2-67330] - Staining IRF1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: IRF1 Antibody (SR44-08) [NBP2-67330] - Analysis of paraffin-embedded mouse brain tissue using anti-IRF1 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
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Western Blot: SATB2 Antibody [NBP1-83619] - Analysis in human cell line HELA.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Staining of human cerebral cortex, colon, liver and rectum using Anti-SATB2 antibody NBP1-83619 (A) shows similar protein distribution across tissues to independent antibody NBP1-92365 (B).
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Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
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Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of whole cell lysate (WCL) of Jurkat cells using anti-Bcl-2 antibody (NB100-56098) at 1:1000 dilution. HRP conjugated goat anti-rabbit IgG (H+L) cross adsorbed secondary antibody was used with ECL substrate for the detection of Bcl2 antibody bound to the blotted protein. This Bcl2 antibody detected a specific band at expected molecular weight marker position of Bcl2 protein (26kDa).
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Western Blot: Caspase-6 Antibody [NB100-56114] - Analysis of Caspase-6 using this antibody. Recombinant catalytically active Caspase-6 was western blotted with Active/Cleaved Caspase-6 antibody. The antibody detected both the large and small subunits of Caspase-6.
Immunohistochemistry-Paraffin: Caspase-6 Antibody [NB100-56114] - Formalin-fixed paraffin-embedded tissue sections of human gliomas stained for Active/Cleaved Caspase-6 expression using Caspase-6 antibody at 1:2000. Gemistocytoma (low grade tumor) at A) low and B) high magnification. Two examples of anaplastic, high grade (III) infiltrating gliomas (C and D). Increased active/cleaved Caspase-6 may be seen in high grade, compared to low grade tumors. Hematoxylin-eosin counterstain.
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(31A1067) - (Pro and ...
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Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of anti-Caspase 3 (31A1067). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Paclitaxel in combination with MWE retarded tumor growth in a human bladder carcinoma TSGH 8301 xenograft model. The levels of total (t-PTEN) and phospho-PTEN (p-PTEN) and Caspase 3 in the tumor specimens were determined by Western blotting and then quantified using beta-actin as the protein loading control; the results are expressed as a percentage of the control. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep20417), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.
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Antibody (SZ04-81)
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Immunocytochemistry/Immunofluorescence: c-Myb [p Ser11] Antibody (SZ04-81) [NBP2-67368] - Staining P-V-Myb+C-Myb(S11) in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: c-Myb [p Ser11] Antibody (SZ04-81) [NBP2-67368] - Analysis of paraffin-embedded human uterine neck tissue using anti-P-V-Myb+C-Myb(S11) antibody. Counter stained with hematoxylin.
Flow Cytometry: c-Myb [p Ser11] Antibody (SZ04-81) [NBP2-67368] - Analysis of MCF-7 cells with P-V-Myb+C-Myb(S11) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as antibody
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