SATB1 Antibody (SP05-03)

Western Blot: SATB1 Antibody (SP05-03) [NBP2-67371] - Analysis of SATB1 on mouse thymus tissue lysates. Predicted band size:86 kDa Observed band size:100 kDa Exposure time:3 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: SATB1 Antibody (SP05-03) [NBP2-67371] - Analysis of paraffin-embedded mouse brain tissue using anti-SATB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: SATB1 Antibody (SP05-03) [NBP2-67371] - Analysis of Jurkat cells with SATB1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Western Blot: SATB1 Antibody (SP05-03) [NBP2-67371] - Analysis of SATB1 on human thymus lysates using anti-SATB1 antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: SATB1 Antibody (SP05-03) [NBP2-67371] - Analysis of paraffin-embedded mouse thymus tissue using anti-SATB1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SATB1 Antibody (SP05-03) [NBP2-67371] - Analysis of paraffin-embedded rat spleen tissue using anti-SATB1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SATB1 Antibody (SP05-03) [NBP2-67371] - Analysis of paraffin-embedded human tonsil tissue using anti-SATB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

SATB1 Antibody (SP05-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SATB1 aa 5-54 / 763. (SwissProt: Q01826 Human; SwissProt: Q60611 Mouse; Unigene: 233782 Rat)
• Localization: Nucleus matrix, PML body.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SATB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SATB1 Antibody (SP05-03)

• DNA-binding protein SATB1
• SATB homeobox 1
• SATB1
• special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's)
• Special AT-rich sequence-binding protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for SATB1 Antibody (NBP2-67371) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 03/07/2022

Summary

• Application: Western Blot
• Sample Tested: Thymus tissue
• Species: Human
• Lot: SP05-03

Comments

• Comments: Human thymus tissue lysate was incubated with the primary antibody overnight in 4 degrees C

Bioinformatics

• Gene Symbol: SATB1