SASS6 Antibody - BSA Free

Western Blot: SASS6 Antibody [NBP2-20264] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE gel, diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: SASS6 Antibody [NBP2-20264] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: SASS6 Antibody [NBP2-20264] - H441 xenograft, using SASS6 antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SASS6 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SASS6 Antibody - BSA Free (NBP2-20264) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SASS6. The exact sequence is proprietary.
• Localization: Centrosome
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SASS6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Chicken (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SASS6 Antibody - BSA Free

• DKFZp761A078
• FLJ22097
• HsSAS-6
• MGC119440
• SAS-6
• SAS6hsSAS-6
• spindle assembly 6 homolog (C. elegans)
• spindle assembly abnormal protein 6 homolog

Background

SAS6 is necessary for centrosome duplication and functions during procentriole formation; SAS6 functions to ensure that each centriole seeds the formation of a single procentriole per cell cycle Strnad et al., (2007) [PubMed 17681132].[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SASS6
• Uniprot:
  • Q6UVJ0(Human)