CEP192 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: CEP192 Antibody [NBP1-84634] - Staining in human testis and pancreas tissues . Corresponding CEP192 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: CEP192 Antibody [NBP1-84634] - Staining of human cell line U-251 MG shows positivity in cytoplasm and centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CEP192 Antibody [NBP1-84634] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity.
Immunohistochemistry-Paraffin: CEP192 Antibody [NBP1-84634] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: CEP192 Antibody [NBP1-84634] - Staining of human pancreas shows few moderate granular cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: CEP192 Antibody [NBP1-84634] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

CEP192 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FVLKERTQENVTLIYNPSDRGINNKTATELSTVYLFGGDEISRQQYRRALLHKPEMIKQILPEHSVLQNINFVEAFQDELLVTEVYD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CEP192
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
WB, ICC/IF reported in scientific literature (PMID: 23932902). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CEP192 Protein (NBP1-84634PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-84634 in the following applications:

Publications
Read Publications using
NBP1-84634 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CEP192 Antibody

  • centrosomal protein 192kDa
  • centrosomal protein of 192 kDa
  • Cep192
  • FLJ10352
  • KIAA1569

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CEP192 Antibody (NBP1-84634)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(2)
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Filter By Species
Human
(2)
All Species

Review for CEP192 Antibody (NBP1-84634) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-84634:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot CEP192 NBP1-84634
Enlarge
4
reviewed by:
Verified Customer
WB Human 08/08/2012
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysates
SpeciesHuman
LotR36766
CommentsDespite the lower unspecific band, provides very nice bands at the predicted molecular weight

Blocking

Blocking Details10% milk TBS-Tween 10 minutes

Primary Anitbody

Dilution Ratio1:500 in 2% BSA TBS-T overnight

Secondary Antibody

Secondary Descriptiongoat-anti-rabbit IgG-HRP
Secondary Manufacturer Cat#Cell Signaling tech 7074
Secondary Concentration1:2000

Details

Detection NotesECL, using siRNA mediated knockdown as negative control

Comments

CommentsDespite the lower unspecific band, provides very nice bands at the predicted molecular weight

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CEP192 Antibody (NBP1-84634) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for CEP192 Antibody (NBP1-84634)

Discover related pathways, diseases and genes to CEP192 Antibody (NBP1-84634). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CEP192 Antibody (NBP1-84634)

Discover more about diseases related to CEP192 Antibody (NBP1-84634).
 

Pathways for CEP192 Antibody (NBP1-84634)

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PTMs for CEP192 Antibody (NBP1-84634)

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Blogs on CEP192

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Recent Reviews

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4
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1
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Verified Customer
08/08/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol CEP192