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SAPS1 Antibody

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Western Blot: SAPS1 Antibody [NBP2-48803] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Western Blot: SAPS1 Antibody [NBP2-48803] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: SAPS1 Antibody [NBP2-48803] - Analysis of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SAPS1 Antibody [NBP2-48803] - Staining of human testis shows strong nuclear and cytoplasmic positivity in Leydig cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SAPS1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SALSANDAALTHELLALDVPNTMLDLFFHYVFNNFLHAQVEGCVSTMLSLGPPPDSSPETPIQNPVVKHLLQQCRLVERILTSWEENDRVQCA
Specificity
Specificity of human SAPS1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP6R1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control
SAPS1 Knockout 293T Cell Lysate
Control Peptide
SAPS1 Recombinant Protein Antigen (NBP2-48803PEP)

Reactivity Notes

Mouse (84%), Rat (84%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SAPS1 Antibody

  • KIAA1115MGC138185
  • PP6R1MGC142003
  • protein phosphatase 6, regulatory subunit 1
  • SAP190
  • SAPS domain family member 1
  • SAPS domain family, member 1
  • SAPS1serine/threonine-protein phosphatase 6 regulatory subunit 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SAPS3 Antibody
NBP2-34048
Immunocytochemistry/Immunofluorescence: SAPS3 Antibody [NBP2-34048] - Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm, plasma membrane & cytosol.
Immunohistochemistry-Paraffin: SAPS3 Antibody [NBP2-34048] - Staining in human parathyroid gland and pancreas tissues using anti-PPP6R3 antibody. Corresponding PPP6R3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SAPS3 Antibody [NBP2-34048] - Staining of human prostate shows strong cytoplasmic and nuclear positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
SCAP2 Antibody
NBP2-47288
Western Blot: SCAP2 Antibody [NBP2-47288] - Analysis in control (vector only transfected HEK293T lysate) and SKAP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: SCAP2 Antibody [NBP2-47288] - Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: SCAP2 Antibody [NBP2-47288] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
SAP155 Antibody
(JB40-32)
NBP2-75656
Western Blot: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of SF3B1 on different lysates using anti-SF3B1 antibody at 1/500 dilution.Positive control:Lane 1: Mouse thymus Lane 2: Hela
Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded rat brain tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded human colon cancer tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
PPP6C Antibody
NBP2-13804
Western Blot: PPP6C Antibody [NBP2-13804] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: PPP6C Antibody [NBP2-13804] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: PPP6C Antibody [NBP2-13804] - Staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.
Species: Hu
Applications: WB, IHC, IHC-P
TMEM115 Antibody
NBP1-80898
Immunohistochemistry-Paraffin: TMEM115 Antibody [NBP1-80898] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
SH2D1A Antibody
(1C9)
H00004068-M01
Western Blot: SH2D1A Antibody (1C9) [H00004068-M01] - Analysis of SH2D1A expression in transfected 293T cell line by SH2D1A monoclonal antibody (M01), clone 1C9.Lane 1: SH2D1A transfected lysate(14.2 KDa).Lane 2: Non-transfected lysate.
Western Blot: SH2D1A Antibody (1C9) [H00004068-M01] - SH2D1A monoclonal antibody (M01), clone 1C9 Analysis of SH2D1A expression in Jurkat.
ELISA: SH2D1A Antibody (1C9) [H00004068-M01] - Detection limit for recombinant GST tagged SH2D1A is 0.3 ng/ml as a capture antibody.
(7 Publications)
Species: Hu, Mu
Applications: WB, ELISA, Flow, Func, S-ELISA
Pentraxin 2/SAP
Antibody [Unconjugate
AF2558
Western blot shows lysates of mouse kidney tissue, mouse liver tissue, and human kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse Pentraxin 2/SAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2558) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pentraxin 2/SAP at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Pentraxin 2/SAP at approximately 36 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Mouse Pentraxin 2/SAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2558) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
(3 Publications)
Species: Hu, Mu
Applications: WB, Simple Western
SAPS2 Antibody
NBP1-88014
Immunocytochemistry/Immunofluorescence: SAPS2 Antibody [NBP1-88014] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: SAPS2 Antibody [NBP1-88014] - Staining of human testis shows moderate cytoplasmic positivity in cells of seminiferus ducts.
Immunohistochemistry-Paraffin: SAPS2 Antibody [NBP1-88014] - Staining of human testis shows moderate cytoplasmic positivity in cells of seminiferus ducts.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
IKBKAP Antibody
(6G9)
H00008518-M03
Western Blot: IKBKAP Antibody (6G9) [H00008518-M03] - Analysis of IKBKAP expression in HeLa (Cat # L013V1).
Immunohistochemistry-Paraffin: IKBKAP Antibody (6G9) [H00008518-M03] - Analysis of monoclonal antibody to IKBKAP on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml
ELISA: IKBKAP Antibody (6G9) [H00008518-M03] - Detection limit for recombinant GST tagged IKBKAP is approximately 0.3ng/ml as a capture antibody.
(2 Publications)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Annexin V
Apoptosis Kit [FITC]
NBP2-29373
Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Controls run before the experimental samples are analyzed, to adjust the fluorescent compensation on the flow cytometer due to the FITC signal from the Annexin V-FITC bleeding into the Propidium Iodide signal.
Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Adherent RAW cells treated with actinomycin-D, detached with a cell detachment solution and stained with the Annexin V Apoptosis Kit [FITC].
(1 Review)
(23 Publications)
Species: Hu, Mu, Rt
ROR
gamma/RORC/NR1F3 Anti
NBP2-24503
Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Total protein from Human and mouse Thymus, Jurkat and U2OS cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ROR Gamma in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - U-2 OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ROR gamma overnight at 4C and detected with an anti-rabbit Dylight 488 (Green). Alpha tubulin (DM1A) NB100-690 was used as a co-stain and detected with an anti-mouse Dylight 550 (Red). Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Tissue section of human lymph node using anti- ROR gamma/RORC/NR1F3 antibody. The staining was developed with HRP labeled secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This antibody generated mainly a nuclear staining in a subset of cells and a weak to cytoplasmic staining was also observed in some cells.
(1 Review)
(2 Publications)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
DNA-PKcs [p
Thr2609] Antibody
NBP1-02456
Western Blot: DNA-PKcs [p Thr2609] Antibody [NBP1-02456] - Detection of a 460 kD band corresponding to human DNA-PKcs in various preparations. Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNA-PKcs inhibitor + IR, Lane 4: MO59J ( DNA-PKcs-) untreated, Lane 5: MO59J IR, Lane 6: Fus1 untreated, Lane 7: Fus1 IR (20Gy, 4h), Lane 8: Fus1 DNA-PKcs inhibitor + IR, Lane 9: MO59J untreated, Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene.
Immunohistochemistry-Paraffin: DNA-PKcs [p Thr2609] Antibody [NBP1-02456] - Staining of human prostate tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
(3 Publications)
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, IHC, IHC-P, IP
IkB-epsilon
Antibody
AF4300
IkB‑ epsilon was detected in immersion fixed paraffin-embedded sections of human astrocytoma using Goat Anti-Human IkB‑ epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4300) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu
Applications: IHC
PP4/PPP4C Antibody
NBP1-50579
Western Blot: PP4/PPP4C Antibody [NBP1-50579] - PP4/PPP4C Antibody (0.3ug/ml) staining of Rat Spleen (A) and Lung (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
Immunohistochemistry-Paraffin: PPP4C Antibody [NBP1-50579] - Analysis of anti-PPP4C antibody with human small intestine at concentration 2.5 ug/ml.
Western Blot: PPP4C Antibody [NBP1-50579] - Analysis of anti-PPP4C antibody with HEK293 lysate (35ug protein in RIPA buffer).
Species: Hu, Mu, Rt, Bt, Bv, Ca, Eq, Ha, Pm, Pm, Rb, Sh, Xp, Ze
Applications: WB, IHC, IHC-P, PEP-ELISA
MTG1 Antibody
NBP2-98486
Western Blot: MTG1 Antibody [NBP2-98486] - Anti-MTG1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 33 kDa
Immunocytochemistry/Immunofluorescence: MTG1 Antibody [NBP2-98486] - Immunofluorescence staining of MTG1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MTG1 polyclonal antibody (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: MTG1 Antibody [NBP2-98486] - Immunochemical staining of human MTG1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Histone H2AX [p
Ser139] Antibody [Unc ...
AF2288
Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μM camptothecin (CPT) for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2288), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Histone H2AX (S139) at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 24 hours, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Histone H2AX (S139) at approximately 25 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2288). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Histone H2AX phosphorylated at S139 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with ultraviolet radiation using Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2288) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(11 Publications)
Species: Hu
Applications: WB, Simple Western, IHC, ICC
p53 Antibody (PAb
240)
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
(3 Reviews)
(27 Publications)
Species: Hu, Mu, Rt, Ch, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
KTI12 Antibody
NBP1-81176
Western Blot: KTI12 Antibody [NBP1-81176] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: KTI12 Antibody [NBP1-81176] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Species: Hu
Applications: WB, IHC, IHC-P
PPP2R4 Antibody
(4D9)
NBP2-37530
Western Blot: PPP2R4 Antibody (4D9) [NBP2-37530] - Western blot analysis using PPP2R4 mAb against human PPP2R4 (AA: 1-154) recombinant protein. (Expected MW is 41 kDa)
Immunocytochemistry/Immunofluorescence: PPP2R4 Antibody (4D9) [NBP2-37530] - Immunofluorescence analysis of Hela cells using PPP2R4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: PPP2R4 Antibody (4D9) [NBP2-37530] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PPP2R4 mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
SAPS1 Antibody
NBP2-48803
SAPS1 Antibody
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

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Product General Protocols

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Control Lysate(s)

SAPS1 Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

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Array NBP2-48803

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Bioinformatics

Gene Symbol PPP6R1