SAP18 Overexpression Lysate (Native)

Images

 
Western Blot: SAP18 Overexpression Lysate (Adult Normal) [NBL1-15685] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SAP18.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SAP18 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205607 and based on accession number NM_005870. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Sin3A-associated protein, 18kDa (SAP18), mRNA.
Gene
SAP18

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
17.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SAP18

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SAP18 Overexpression Lysate (Native)

  • 2HOR0202cell growth inhibiting protein 38
  • Cell growth-inhibiting gene 38 protein
  • cell growth-inhibiting protein 38
  • histone deacetlyase complex subunit SAP18
  • histone deacetylase complex subunit SAP18,18 kDa Sin3-associated polypeptide
  • MGC27131
  • SAP18P
  • Sin3A-associated protein, 18kDa
  • Sin3-associated polypeptide p18
  • sin3-associated polypeptide, 18 kDa
  • sin3-associated polypeptide, p18

Background

Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SAP18