SAP155 Antibody

Western Blot: SAP155 Antibody [NBP2-47291] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.

Immunocytochemistry/Immunofluorescence: SAP155 Antibody [NBP2-47291] - Immunofluorescent staining of human cell line HeLa shows localization to nuclear speckles.

Immunohistochemistry-Paraffin: SAP155 Antibody [NBP2-47291] - Immunohistochemical staining of human salivary gland shows strong nuclear and cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SAP155 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: LDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADRED
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SF3B1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SAP155 Protein (NBP2-47291PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SAP155 Antibody

• Hsh155
• pre-mRNA processing 10
• pre-mRNA splicing factor SF3b, 155 kDa subunit
• Pre-mRNA-splicing factor SF3b 155 kDa subunit
• Prp10
• PRPF10
• SAP 155
• SAP155splicing factor 3b, subunit 1, 155kD
• SF3B155
• SF3b155PRP10
• spliceosome associated protein 155
• Spliceosome-associated protein 155
• splicing factor 3B subunit 1
• splicing factor 3b, subunit 1, 155kDa

Background

SF3B155 is a subunit of the U2 small nuclear ribonucleoproteins complex (U2 snRNP) that functions in pre-mRNA splicing. SF3B155 together with splicing factor 3a binds pre-mRNA upstream of the branchpoint sequence to anchor U2 snRNP to the pre-mRNA. SF3B155 is also a component of the U12-type splicesome that is involved in the splicing of a rare class of nuclear pre-mRNA introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SF3B1