SAP155 Antibody

Images

 
Independent Antibodies: Western Blot: SAP155 Antibody [NB100-55256] - Detection of Human SF3b155/SAP155 on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lysate (50 microgram) using Nb100-55256. ...read more
Immunohistochemistry-Paraffin: SAP155 Antibody [NB100-55256] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SF3b155/SAP155 used at a dilution of 1:200 (1ug/ml). Detection: ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

SAP155 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human splicing factor 3b, subunit 1, 155kDa using the numbering given in entry NP_036565.2 (GeneID 23451).
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SF3B1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
This antibody is useful for Western Blot and Immunoprecipitation, recognizes a ~155 kDa band. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
146 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB100-55256.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SAP155 Antibody

  • Hsh155
  • pre-mRNA processing 10
  • pre-mRNA splicing factor SF3b, 155 kDa subunit
  • Pre-mRNA-splicing factor SF3b 155 kDa subunit
  • Prp10
  • PRPF10
  • SAP 155
  • SAP155splicing factor 3b, subunit 1, 155kD
  • SF3B155
  • SF3b155PRP10
  • spliceosome associated protein 155
  • Spliceosome-associated protein 155
  • splicing factor 3B subunit 1
  • splicing factor 3b, subunit 1, 155kDa

Background

SF3B155 is a subunit of the U2 small nuclear ribonucleoproteins complex (U2 snRNP) that functions in pre-mRNA splicing. SF3B155 together with splicing factor 3a binds pre-mRNA upstream of the branchpoint sequence to anchor U2 snRNP to the pre-mRNA. SF3B155 is also a component of the U12-type splicesome that is involved in the splicing of a rare class of nuclear pre-mRNA introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SAP155 Antibody (NB100-55256)(1)


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Bioinformatics

Gene Symbol SF3B1
Entrez
Uniprot