SAP130 Antibody (2F1)

Immunocytochemistry/Immunofluorescence: SAP130 Antibody (2F1) [H00023450-M03] - Immunofluorescence of monoclonal antibody to SAP130 on HeLa cell. [antibody concentration 40 ug/ml]

Immunocytochemistry/Immunofluorescence: SAP130 Antibody (2F1) [H00023450-M03] - Analysis of monoclonal antibody to SF3B3 on HeLa cell. Antibody concentration 40 ug/ml

Sandwich ELISA: SAP130 Antibody (2F1) [H00023450-M03] - Detection limit for recombinant GST tagged SF3B3 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: ELISA, ICC/IF, S-ELISA
• Clone: 2F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SAP130 Antibody (2F1) Summary

• Immunogen: SF3B3 (NP_036558.3, 819 a.a. ~ 929 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVAKDLILNPRSVAGGFVYTYK
• Specificity: SF3B3 - splicing factor 3b, subunit 3, 130kDa (2F1)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SF3B3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Sandwich ELISA
• Application Notes: This product is useful for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SAP130 Antibody (2F1)

• KIAA0017splicing factor 3b, subunit 3, 130kD
• Pre-mRNA-splicing factor SF3b 130 kDa subunit
• RSE1
• SAP130SAP 130
• SF3B130
• SF3b130pre-mRNA splicing factor SF3b, 130 kDa subunit
• Spliceosome-associated protein 130
• splicing factor 3B subunit 3
• splicing factor 3b, subunit 3, 130kDa
• STAF130

Background

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SF3B3
• Entrez:
  • 23450(Human)
• Uniprot:
  • NP_036558(Human)