SAP102 Antibody

Western Blot: SAP102 Antibody [NB100-2063] - Analysis of SAP102 on extract from COS cells transfected with rat SAP102.

Immunohistochemistry-Frozen: SAP102 Antibody [NB100-2063] - Staining of Ammon's Horn and the dentate gyrus of the rat hippocampus.

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

SAP102 Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues G(83) P V P P K P V P G K N T P K L N G(100) of rat SAP102.
• Localization: Synaptic membrane.
• Specificity: SAP102
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DLG3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 10 ug/ml
  • Western Blot 3 ug/ml
• Application Notes: May be Useful in WB. WB: Detects an approx. 102 kDa protein representing SAP 102 in rat brain homogenate. IHC: Staining of SAP 102 in rat hippocampus yields a pattern consistent with post-synaptic localization.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 12438413).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SAP102 Antibody

• discs, large homolog 3 (Drosophila)
• KIAA1232discs, large homolog 3 (neuroendocrine-dlg, Drosophila)
• MRX90MRX
• NEDLGdisks large homolog 3
• neuroendocrine-DLG
• SAP-102
• SAP102NE-Dlg
• Synapse-associated protein 102
• XLMR

Background

Synapse-Associated Protein 102 (SAP102) is one of a family of plasma membrane-associated proteins found in synaptic junctions. Like other members of the family, SAP102 has three ~90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity. SAP102 is believed to participate in the clustering of certain proteins, including NMDA receptors, shaker-type potassium channels at the synaptic membrane in CNS neurons. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP102 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP102 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLG3
• Entrez:
  • 1741(Human)
  • 53310(Mouse)
  • 58948(Rat)