SAMM50 Antibody - BSA Free

Western Blot: SAMM50 Antibody [NBP3-06283] - Anti-SAMM50 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP3-06283] - Immunochemical staining of human SAMM50 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: SAMM50 Antibody [NBP3-06283] - SAMM50 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-SAMM50 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody:Anti-SAMM50 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 50 kDaObserved band size :55 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SAMM50 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human SAMM50 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SAMM50
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for SAMM50 Antibody - BSA Free

• CGI-51
• FLJ42905
• FLJ99036
• OMP85
• SAM50
• sorting and assembly machinery 50kDa
• sorting and assembly machinery component 50 homolog (S. cerevisiae)
• sorting and assembly machinery component 50 homolog
• TOB55
• Transformation-related gene 3 protein
• transformation-related protein 3
• TRG-3FLJ35825
• YNL026W

Background

SAMM50 is a component of the sorting and assembly machinery (SAM) complex of the outer mitochondrial membrane. The SAMcomplex has a role in integrating beta-barrel proteins into the outer mitochondrial membrane (Humphries et al., 2005(PubMed 15644312)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SAMM50