SAMM50 Antibody - BSA Free

Western Blot: SAMM50 Antibody [NBP2-94852] - Analysis of extracts of various cell lines, using SAMM50 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP2-94852] - Immunohistochemistry of paraffin-embedded mouse heart using SAMM50 Rabbit pAb (NBP2-94852) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP2-94852] - Immunohistochemistry of paraffin-embedded rat heart using SAMM50 Rabbit pAb (NBP2-94852) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP2-94852] - Immunohistochemistry of paraffin-embedded human liver cancer using SAMM50 Rabbit pAb (NBP2-94852) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunoprecipitation: SAMM50 Antibody [NBP2-94852] - Immunoprecipitation analysis of 600ug extracts of Mouse heart cells using 3ug SAMM50 antibody (NBP2-94852). Western blot was performed from the immunoprecipitate using SAMM50 antibody (NBP2-94852) at a dilution of 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SAMM50 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SAMM50 Antibody - BSA Free (NBP2-94852) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SAMM50 (NP_056195.3). MGTVHARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLIDTCQGDDALPNGLDVTFEVTELRRLTGSYNTMVGNNEGSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPRPGNFERNFSVNLYKVTGQFPWSSLRETDRGMSAEYSFPIW
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SAMM50
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:500 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SAMM50 Antibody - BSA Free

• CGI-51
• FLJ42905
• FLJ99036
• OMP85
• SAM50
• sorting and assembly machinery 50kDa
• sorting and assembly machinery component 50 homolog (S. cerevisiae)
• sorting and assembly machinery component 50 homolog
• TOB55
• Transformation-related gene 3 protein
• transformation-related protein 3
• TRG-3FLJ35825
• YNL026W

Background

SAMM50 is a component of the sorting and assembly machinery (SAM) complex of the outer mitochondrial membrane. The SAMcomplex has a role in integrating beta-barrel proteins into the outer mitochondrial membrane (Humphries et al., 2005(PubMed 15644312)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SAMM50