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S1P4/EDG-6 Antibody

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Western Blot: S1P4/EDG-6 Antibody [NBP2-24500] - Analysis of EDG6 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW using NBP2-24500 at 4.0 ug/ml.
Western Blot: S1P4/EDG-6 Antibody [NBP2-24500] - Analysis of EDG6 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW using NBP2-24500 at 4.0 ug/ml.
Immunohistochemistry-Paraffin: S1P4/EDG-6 Antibody [NBP2-24500] - Analysis of Edg6/S1P4 in paraffin-embedded formalin-fixed mouse RAW cells using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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S1P4/EDG-6 Antibody Summary

Immunogen
A portion of amino acids 1-50 of human Sphingolipid Receptor Edg6/S1P4 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
S1PR4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 3.0-5.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry-Paraffin

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human and 95% homologous in mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for S1P4/EDG-6 Antibody

  • EDG6
  • EDG6S1P receptor Edg-6
  • Endothelial differentiation G-protein coupled receptor 6
  • endothelial differentiation, G-protein-coupled receptor 6
  • endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor6,endothelial differentiation, G protein coupled receptor 6
  • S1P receptor 4
  • S1P4
  • S1P4LPC1
  • S1PR4
  • SLP4
  • sphingosine 1-phosphate receptor 4
  • Sphingosine 1-phosphate receptor Edg-6
  • sphingosine-1-phosphate receptor 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG‑1 and GFP was stained with (A) Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A isotype control antibody (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
S1P1/EDG‑1 was detected in perfusion fixed frozen sections of mouse heart using Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter­stained with hematoxylin (blue). Specific staining was localized to endothelial cells in capillaries. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
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MBTPS1 Antibody
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Immunohistochemistry: MBTPS1 Antibody [NBP2-49214] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Species: Hu
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S1P3/EDG-3 Antibody
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Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Human neuroblastoma with various drugs and control. Image from verified customer review.
Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP2-24762] - EDG3 in formalin-fixed, paraffin-embedded human liver tissue using an isotype control (top left) and NBP2-24762 (bottom left, right) at 5 ug/ml.
Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Sphingolipid Receptor Edg3/S1P3 in kidney lysate. Lane 1 shows pre-immune sera. Lanes 2 and 3 show NBP2-24762 tested on human kidney (2 ug/ml) and mouse kidney (1 ug/ml) lysate.
Species: Hu, Mu
Applications: WB, IHC-P
S1P2/EDG-5/S1PR2
Antibody
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Western Blot: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Analysis of S1P2/Edg5 in heart lysate; Lane 1 shows pre-immune sera; Lanes 2, 3 and 4 show this antibody tested on human heart (1 ug/ml), mouse heart (1 ug/ml) and rat heart (2 ug/ml) lysate, respectively.
Immunocytochemistry/Immunofluorescence: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Human PC3 prostate cancer cell line. Primary antibody NBP2-26691 at 1:200, secondary antibody Alexa488, Hoechst 33342 used for nuclear staining. Image from verified customer review.
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Western Blot: S1P5/EDG-8 Antibody [NBP2-24712] - Analysis of EDG8 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse, 4) rat using NBP2-24712 at 5 ug/ml, 2 ug/ml and 2 ug/ml.
Immunohistochemistry-Paraffin: S1P5/EDG-8 Antibody [NBP2-24712] - Staining of normal human brain using NBP2-24712 at 5 ug/ml.
Immunohistochemistry-Paraffin: S1P5/EDG-8 Antibody [NBP2-24712] - Staining of normal human brain using control (rabbit Ig) at 5 ug/ml.
(1 Publication)
Species: Hu, Mu, Rt, Ca, Pm, Xp
Applications: WB, IHC-P
LPAR1/LPA1/EDG-2
Antibody
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Western Blot: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - Detection of EDG2 in A549 whole cell extracts using NBP1-03363. The band at ~41 kDa position represents the target protein EDG2, whereas, the band at ~55kDa may potentially be the post-translationally modified (glycosylated, palmitoylated or lipidated) form of this protein.
Immunocytochemistry/Immunofluorescence: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-EDG2/LPAR1/LPA1 (NBP1-03363) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human prostate cancer using LPAR1 antibody at 1:100 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was obseved in the scattered ducts. Staining was performed by Histowiz.
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Immunohistochemistry-Paraffin: LPAR2/LPA2/EDG-4 Antibody [NLS1014] - Human Colon, Carcinoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: LPAR2/LPA2/EDG-4 Antibody [NLS1014] - Analysis of anti-EDG7 antibody with human brain, amygdala.
Immunohistochemistry-Paraffin: LPAR2/LPA2/EDG-4 Antibody [NLS1014] - Human heart, diabetes formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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LPAR2/LPA2/EDG-4
Antibody
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Western blot shows lysates of human peripheral blood lymphocytes (PBL). PVDF Membrane was probed with 1 µg/mL of Human LPAR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6396) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LPAR2 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Lipoprotein a
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NBP2-37477
Western Blot: Lipoprotein a Antibody (4H1) [NBP2-37477] - Western blot analysis using LPA mAb against human LPA recombinant protein. (Expected MW is 34.1 kDa)
Immunocytochemistry/Immunofluorescence: Lipoprotein a Antibody (4H1) [NBP2-37477] - Immunofluorescence analysis of HepG2 cells using LPA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: Lipoprotein a Antibody (4H1) [NBP2-37477] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using LPA mouse mAb with DAB staining.
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Hexim 1 Antibody
NBP1-71867
Western Blot: Hexim 1 Antibody [NBP1-71867] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HEXIM1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71866 and NBP1-71868, which recognize other epitopes.
Immunohistochemistry: Hexim 1 Antibody [NBP1-71867] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml) ( ) or 1:1,000 (1ug/ml) ( and ). Detection: DAB. Counterstain: hematoxylin (blue).
Immunohistochemistry: Hexim 1 Antibody [NBP1-71867] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).
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Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
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IL-2 [Unconjugated]
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Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
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SYTL4 Antibody
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Western Blot: SYTL4 Antibody [NBP1-86176] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: SYTL4 Antibody [NBP1-86176] - Staining of human cell line A-431 shows localization to nucleoplasm, cytosol & microtubule organizing center.
Immunohistochemistry-Paraffin: SYTL4 Antibody [NBP1-86176] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
(2 Publications)
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Rhodopsin Antibody
(A531)
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Western Blot: Rhodopsin Antibody (A531) [NBP2-25159] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hydrophobic nature of Rhodopsin, it is important not to boil a sample containing it in SDS-PAGE sample buffer, as this will result in more extensive aggregation of the Rhodopsin protein.
Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (A531) [NBP2-25159] - Pig retinal section stained with NBP2-25159 (green) and counterstained with rabbit polyclonal antibody to NF-M NB300-133 (red) and DNA (blue). Rhodopsin is most abundant in the outer segments of retina (OS), NF-M is abundant in the optic nerve fiber layer (ONFL), but seen in processes and neurons in other regions also. Other layers are pigmented epithelium (PE), outer and inner nuclear layers (ONL, INL), outer and inner plexiform layers (OPL, IPL) and ganglion cell layer (GCL).
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RhoD Antibody
NBP1-58359
Western Blot: RhoD Antibody [NBP1-58359] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry: RhoD Antibody [NBP1-58359] - Human Liver Tissue Observed Staining: Cytoplasm in sinusoids of liver Primary Antibody Concentration: 1 : 100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Expos
Immunohistochemistry-Paraffin: RhoD Antibody [NBP1-58359] - Human Tonsil Tissue, 4-8ug/ml.
Species: Hu
Applications: WB, IHC, IHC-P
BUD31 Antibody
NBP1-84010
Western Blot: BUD31 Antibody [NBP1-84010] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: BUD31 Antibody [NBP1-84010] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & microtubules.
Immunohistochemistry-Paraffin: BUD31 Antibody [NBP1-84010] - Staining of human nasopharynx shows strong cytoplasmic and nuclear positivity in respiratory epithelial cells.
Species: Hu, Mu, Rt
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CD4 Antibody
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Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: Lane 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.
(7 Reviews)
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(1G7)
H00005873-M02
Western Blot: Rab27a Antibody (1G7) [H00005873-M02] - RAB27A monoclonal antibody (M02), clone 1G7 Analysis of RAB27A expression in HL-60.
Immunohistochemistry-Paraffin: Rab27a Antibody (1G7) [H00005873-M02] - Analysis of monoclonal antibody to RAB27A on formalin-fixed paraffin-embedded human lymphoma. Antibody concentration 3 ug/ml.
Western Blot: Rab27a Antibody (1G7) [H00005873-M02] - Analysis of RAB27A over-expressed 293 cell line, cotransfected with RAB27A Validated Chimera RNAi ( Cat # H00005873-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAB27A monoclonal antibody (M02), clone 1G7 (Cat # H00005873-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
(12 Publications)
Species: Hu
Applications: WB, ELISA, IHC-P, RNAi
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Bioinformatics

Gene Symbol S1PR4
Entrez
Uniprot