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S1P4/EDG-6 Antibody

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Western Blot: S1P4/EDG-6 Antibody [NBP2-24500] - Analysis of EDG6 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW using NBP2-24500 at 4.0 ug/ml.
Western Blot: S1P4/EDG-6 Antibody [NBP2-24500] - Analysis of EDG6 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW using NBP2-24500 at 4.0 ug/ml.
Immunohistochemistry-Paraffin: S1P4/EDG-6 Antibody [NBP2-24500] - Analysis of Edg6/S1P4 in paraffin-embedded formalin-fixed mouse RAW cells using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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S1P4/EDG-6 Antibody Summary

Immunogen
A portion of amino acids 1-50 of human Sphingolipid Receptor Edg6/S1P4 was used as the immunogen.
Predicted Species
Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
S1PR4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 3.0-5.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Publications
Read Publication using
NBP2-24500 in the following applications:

  • WB
    1 publication

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: 100% homologous in human; mouse (95%), rat (95%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for S1P4/EDG-6 Antibody

  • EDG6
  • EDG6S1P receptor Edg-6
  • Endothelial differentiation G-protein coupled receptor 6
  • endothelial differentiation, G-protein-coupled receptor 6
  • endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor6,endothelial differentiation, G protein coupled receptor 6
  • S1P receptor 4
  • S1P4
  • S1P4LPC1
  • S1PR4
  • SLP4
  • sphingosine 1-phosphate receptor 4
  • Sphingosine 1-phosphate receptor Edg-6
  • sphingosine-1-phosphate receptor 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: MBTPS1 Antibody [NBP3-05160] - Paraffin-embedded human colon using MBTPS1 antibody at dilution of 1:100 (40x lens).
Immunocytochemistry/Immunofluorescence: MBTPS1 Antibody [NBP3-05160] - Analysis of U2OS cells using MBTPS1 antibody. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: MBTPS1 Antibody [NBP3-05160] - Paraffin-embedded human stomach using MBTPS1 antibody at dilution of 1:100 (40x lens).
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Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Human neuroblastoma with various drugs and control. Image from verified customer review.
Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP2-24762] - Human liver tissue using an isotype control (top left) and NBP2-24762 (bottom left, right) at 5 ug/ml.
Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Sphingolipid Receptor Edg3/S1P3 in kidney lysate. Lane 1 shows pre-immune sera. Lanes 2 and 3 show NBP2-24762 tested on human kidney (2 ug/ml) and mouse kidney (1 ug/ml) lysate.
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Western Blot: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Analysis of S1P2/Edg5 in heart lysate; Lane 1 shows pre-immune sera; Lanes 2, 3 and 4 show this antibody tested on human heart (1 ug/ml), mouse heart (1 ug/ml) and rat heart (2 ug/ml) lysate, respectively.
Immunocytochemistry/Immunofluorescence: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Human PC3 prostate cancer cell line. Primary antibody NBP2-26691 at 1:200, secondary antibody Alexa488, Hoechst 33342 used for nuclear staining. Image from verified customer review.
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Western Blot: S1P5/EDG-8 Antibody [NBP2-24712] - Analysis of EDG8 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse, 4) rat using NBP2-24712 at 5 ug/ml, 2 ug/ml and 2 ug/ml.
Immunohistochemistry-Paraffin: S1P5/EDG-8 Antibody [NBP2-24712] - Staining of normal human brain using NBP2-24712 at 5 ug/ml.
Immunohistochemistry-Paraffin: S1P5/EDG-8 Antibody [NBP2-24712] - Staining of normal human brain using control (rabbit Ig) at 5 ug/ml.
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Western Blot: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - Detection of EDG2 in A549 whole cell extracts using NBP1-03363. The band at ~41 kDa position represents the target protein EDG2, whereas, the band at ~55kDa may potentially be the post-translationally modified (glycosylated, palmitoylated or lipidated) form of this protein.
Immunocytochemistry/Immunofluorescence: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-EDG2/LPAR1/LPA1 (NBP1-03363) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human prostate cancer using LPAR1 antibody at 1:100 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was obseved in the scattered ducts. Staining was performed by Histowiz.
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Immunocytochemistry/Immunofluorescence: LPAR3/LPA3/EDG-7 Antibody [NBP1-84903] - Staining of human cell line PC-3 shows localization to plasma membrane. Antibody staining is shown in green.
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Immunohistochemistry-Paraffin: LPAR2/LPA2/EDG-4 Antibody [NBP1-84904] - Staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.
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Western Blot: Lipoprotein a Antibody (4H1) [NBP2-37477] - Western blot analysis using LPA mAb against human LPA recombinant protein. (Expected MW is 34.1 kDa)
Immunocytochemistry/Immunofluorescence: Lipoprotein a Antibody (4H1) [NBP2-37477] - Immunofluorescence analysis of HepG2 cells using LPA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Western Blot: Hexim 1 Antibody [NBP1-71867] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HEXIM1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71866 and NBP1-71868, which recognize other epitopes.
Immunohistochemistry: Hexim 1 Antibody [NBP1-71867] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).
Immunohistochemistry: Hexim 1 Antibody [NBP1-71867] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml) ( ) or 1:1,000 (1ug/ml) ( and ). Detection: DAB. Counterstain: hematoxylin (blue).
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Western Blot: ERK2 Antibody (6E5) [NBP1-47842] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ERK2 monoclonal antibody.
Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Endometrium tissue within the normal limits using antiMAPK1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,Dilution 1:50)
Western Blot: ERK2 Antibody (6E5) [NBP1-47842] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERK2.
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Western Blot: SYTL4 Antibody [NBP1-86174] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: SYTL4 Antibody [NBP1-86174] - Staining of human cell line A-431 shows localization to nucleoplasm, cytosol & microtubule organizing center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SYTL4 Antibody [NBP1-86174] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.
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Western Blot: Rhodopsin Antibody (B630) [NBP2-25160] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hydrophobic nature of Rhodopsin, it is important to avoid boiling samples containing this protein in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the Rhodopsin protein and appearance of more of this high molecular weight material.
Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (B630) [NBP2-25160] - High magnification confocal image of pig retinal section stained with NBP2-25160 (Green). Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).
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Western Blot: RhoD Antibody [NBP1-58359] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry: RhoD Antibody [NBP1-58359] - Human Liver Tissue Observed Staining: Cytoplasm in sinusoids of liver Primary Antibody Concentration: 1 : 100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Expos
Immunohistochemistry-Paraffin: RhoD Antibody [NBP1-58359] - Human Tonsil Tissue, 4-8ug/ml.
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Western Blot: BUD31 Antibody [NBP1-84010] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: BUD31 Antibody [NBP1-84010] - Staining of human cell line U-2 OS shows localization to nucleus & microtubules. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BUD31 Antibody [NBP1-84010] - Staining of human nasopharynx shows strong cytoplasmic and nuclear positivity in respiratory epithelial cells.
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Immunohistochemistry: CD4 Antibody [NBP1-19371] - Increased CD3+ and CD4+ T-cell occurrence in the brainstem of SHR-72 transgenic rat model for tauopathies. (A-D) Immunofluorescence staining showed CD4+ T-cells in SHR-72 transgenic animals. (E- H) Immunofluorescence staining showed more perivascular than brain parenchyma infiltrating CD4+ T-cells in SHR-72 transgenic animals. PLoS One. 2019 May 23;14(5):e0217216. doi: 10.1371/journal.pone.0217216.
Immunohistochemistry: CD4 Antibody [NBP1-19371] - DBZ inhibits the accumulation of CD4+ T cells and Th2 differentiation in the AAAs. (B) The representation of immunohistochemical staining for CD4+ in abdominal aorta from four groups (left). Bar graphs show the percentage of CD4+ positive cell areas (right; n=3 per group). Bar: 50 um.
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
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Western Blot: Rab27a Antibody (1G7) [H00005873-M02] - Analysis of RAB27A expression in transfected 293T cell line by RAB27A monoclonal antibody (M02), clone 1G7.Lane 1: RAB27A transfected lysate(24.9 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Rab27a Antibody (1G7) [H00005873-M02] - Analysis of monoclonal antibody to RAB27A on formalin-fixed paraffin-embedded human lymphoma. Antibody concentration 3 ug/ml.
Western Blot: Rab27a Antibody (1G7) [H00005873-M02] - Analysis of RAB27A over-expressed 293 cell line, cotransfected with RAB27A Validated Chimera RNAi ( Cat # H00005873-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAB27A monoclonal antibody (M02), clone 1G7 (Cat # H00005873-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Publications for S1P4/EDG-6 Antibody (NBP2-24500)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Showing Publication 1 - 1 of 1.
Publication using NBP2-24500 Applications Species
Lee K, Koh E, Hong C et al. A novel S1PR4 functional antagonist prevents nonalcoholic steatohepatitis by deactivating the NLRP3 inflammasome without causing lymphopenia Research Square Nov 13 2020 (WB, Mouse) WB Mouse

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Bioinformatics

Gene Symbol S1PR4
Uniprot