S1P1/EDG-1 Antibody

Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Gene and protein expression of components of the PAR-1/SphK/S1P axis. Western blot detection and quantification of the protein expression of PAR-1, S1PR1, SphK1, and SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for 6 h. The data are presented as the mean +/- SD (n = 3), *p < 0.05. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fnmol.2020.00114/full) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NB120-11424] - Immunofluorescence of PAR-1 and S1PR1 in spinal cord tissues of mice induced by EAE. Mice subjected to EAE modeling were treated with vehicle (physiological saline), Dab, or PAR-1-inh. Tissues were stained for (B) S1PR1 in green and nuclei (DAPI) in blue. Scale bar = 100 um. Green fluorescence was quantified by measuring the relative mean IOD. The data are presented as the mean +/- SD (n = 6), *p < 0.05. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/articles/10.3389/fnmol.2020.00114/full) licensed under a CC-BY license.

Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Analysis of S1P1 from transfected HEK293 cell lysate.

Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NB120-11424] - Immunofluorescence staining of S1P1 in transfected CCL39 cells using NB120-11424.

Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NB120-11424] - Immunofluorescence of proteins related to S1P1/EDG-1 signaling in astrocytes. Astrocytes were treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only) for 6 h. Cells were stained for S1P1/EDG-1 receptor 1 in green and nuclei (DAPI) in blue. Scale bar = 100 um. Green fluorescence was quantified by measuring the relative mean IOD. The data are presented as the mean +/- SD (n = 3), *p < 0.05. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fnmol.2020.00114/full) licensed under a CC-BY license.

Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Gene & protein expression of components of the PAR-1/SphK/S1P axis. qRT-PCR analysis of the gene expression of (A) PAR-1, (B) S1PR1, (C) SphK1, & (D) SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for 0 h, 1 h, or 6 h. Western blot detection & quantification of the protein expression of PAR-1, S1PR1, SphK1, & SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for (E) 0 h, (F) 1 h, or (G) 6 h. The data are presented as the mean ± SD (n = 3), *p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32694981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Expression of sphingosine-1-phosphate (S1P), interleukin (IL)-1 beta , & protease-activated receptor-1 (PAR-1) in isolated astrocytes. (A) Isolated & purified astrocytes were identified by bright-field microscopy & immunofluorescence for glial fibrillary acid protein (GFAP; red). Nuclei (DAPI) were labeled in blue. Scale bar = 100 μm. (B) Western blot & quantitative reverse transcription polymerase chain reaction (qRT-PCR) of the protein & mRNA expression, respectively, of S1P 0, 1, & 6 h after astrocytes were treated with lipopolysaccharide (LPS) or thrombin & Dabigatran (Dab) or PAR-1-inh (LPS only). (D) qRT-PCR & (E) enzyme-linked immunosorbent assay (ELISA) of the mRNA expression & secretion, respectively, of IL-1 beta 0, 1, & 6 h after astrocytes were treated with LPS or thrombin & Dab or PAR-1-inh (LPS only). (F) Immunofluorescence of astrocytes & quantification of relative mean integrated optical density (IOD; green fluorescence). PAR-1 was stained in green & nuclei (DAPI) were stained in blue. Scale bar = 100 μm. For (B–F), data are presented as the mean ± SD (n = 3). *p < 0.05; #p < 0.05 vs. the same group at 0 h; &p < 0.05 vs. the same group at 1 h. ns, not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32694981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

S1P1/EDG-1 Antibody Summary

• Description: Novus Biologicals Rabbit S1P1/EDG-1 Antibody (NB120-11424) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-S1P1/EDG-1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1.
• Localization: Cell Membrane
• Specificity: NB120-11424 detects S1P1 protein (EDG1) in transfected human cell samples. This shows no cross-reactivity to S1P3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: S1PR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2 ug/mL
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: This antibody has successfully been used in immunofluorescence, immunohistochemistry, immunoprecipitation and Western blot procedures. By Western blot, this antibody detects an ~44 kDa protein representing recombinant S1P1 from transfected HEK293 cells. Immunofluorescence data demonstrates that S1P1 is localized to the plasma membrane after staining with NB120-11424.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1 mg/mL BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for S1P1/EDG-1 Antibody

• CD363 antigen
• CD363
• CHEDG1
• D1S3362
• EDG1
• EDG-1
• EDG1edg-1
• Endothelial differentiation G-protein coupled receptor 1
• endothelial differentiation, sphingolipid G-protein-coupled receptor, 1
• FLJ58121
• S1P receptor 1
• S1P receptor Edg-1
• S1P1
• S1P1ECGF1
• S1PR1
• sphingosine 1-phosphate receptor 1
• sphingosine 1-phosphate receptor EDG1
• Sphingosine 1-phosphate receptor Edg-1
• sphingosine-1-phosphate receptor 1

Background

S1P1 is a G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that is coupled to the G(i) subclass of heteromeric G proteins. S1P1 signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. It plays an important role in cell migration via its role in the reorganization of the actin cytoskeleton and formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. S1P1 is required for normal chemotaxis toward sphingosine 1-phosphate, normal embryonic heart development, and normal cardiac morphogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S1P1/EDG-1 Antibody (NB120-11424)(4)

Publications using NB120-11424

• Goel K, Schweitzer KS, Serban KA et al. Pharmacological sphingosine-1 phosphate receptor 1 targeting in cigarette smoke-induced emphysema in mice American journal of physiology. Lung cellular and molecular physiology 2022-04-12 [PMID: 35412858] (IF/IHC, Mouse)
• Goel K, Beatman E, Egersdorf N et al. Sphingosine 1 Phosphate (S1P) Receptor 1 Is Decreased in Human Lung Microvascular Endothelial Cells of Smokers and Mediates S1P Effect on Autophagy Cells 2021-05-14 [PMID: 34068927] (ICC/IF, IHC-P, WB, Human, Mouse)
• Chen R, Cao X, et al. Dabigatran Suppresses PAR-1/SphK/S1P Activation of Astrocytes in Experimental Autoimmune Encephalomyelitis Model. Front Mol Neurosci 2020-07-23 [PMID: 32694981] (ICC/IF, WB, Mouse)
• Awojoodu AO, Ogle ME, Sefcik LS et al. Sphingosine 1-phosphate receptor 3 regulates recruitment of anti-inflammatory monocytes to microvessels during implant arteriogenesis. Proc Natl Acad Sci U S A. 2013-08-20 [PMID: 23918395] (WB, Mouse)

Bioinformatics

• Gene Symbol: S1PR1
• Entrez:
  • 1901(Human)
  • 13609(Mouse)
  • 29733(Rat)
• Uniprot:
  • O08530()