S1P1/EDG-1 Antibodies
We offer S1P1/EDG-1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each S1P1/EDG-1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our S1P1/EDG-1 Antibodies can be used in a variety of model species: Hamster, Human, Mouse, Rat, Zebrafish. Use the list below to choose the S1P1/EDG-1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our S1P1/EDG-1 polyclonal antibodies and browse our S1P1/EDG-1 monoclonal antibody catalog.
Alternate Names for S1P1/EDG-1 Antibodies
anti-S1P1/EDG-1 antibody, anti-S1PR1 antibody, anti-CD363 antibody, anti-CD363 antigen antibody, anti-CHEDG1 antibody, anti-D1S3362 antibody, anti-EDG-1 antibody, anti-EDG1edg-1 antibody, anti-Endothelial differentiation G-protein coupled receptor 1 antibody, anti-endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 antibody, anti-FLJ58121 antibody, anti-S1P receptor 1 antibody, anti-S1P receptor Edg-1 antibody, anti-s1p1 antibody, anti-S1P1ECGF1 antibody, anti-sphingosine 1-phosphate receptor 1 antibody, anti-Sphingosine 1-phosphate receptor Edg-1 antibody, anti-sphingosine 1-phosphate receptor EDG1 antibody, anti-sphingosine-1-phosphate receptor 1 antibody
13 Results for "s1p1-edg-1" in Primary Antibodies
MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and GFP was stained with (A) Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A isotype control antibody (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." alt="CHO Chinese hamster ovary cell line transfected with either (A) human S1P1/EDG-1 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB2016) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A Isotype Control (Catalog # 
CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to endothelial cells in capillaries. View our protocol for ![CD69 and S1PR1 expression on CD4 T cells before and following cell transfer. Activated OT-II T cells were analyzed before cell transfer (A) for expression of CD69 and S1PR1 or for CD69 expression ex vivo 24 h after transfer into resting (PBS) or inflamed [lipopolysaccharide (LPS)] ear pinnae (B,C). Before transfer, the OT-II T cells were treated with an S1PR1 antagonist or agonist as in Figure 5B. In panel (A), cells are gated on live CD4+ CD45+ cells; representative of three independent samples. In panels (B,C), data are combined from two to three independent experiments with ≥3 animals per group. In this figure, p < 0.05 is indicated by * and p < 0.001 by **. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29225602), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab7089_mouse-s1p1-edg-1-mab-clone-713412-flow-cytometry-12122025753480.jpg)
![Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Analysis of S1P1 from transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Western-Blot-NB120-11424-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NB120-11424] - Immunofluorescence staining of S1P1 in transfected CCL39 cells using NB120-11424.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11424-img0004.jpg)
![Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Gene & protein expression of components of the PAR-1/SphK/S1P axis. qRT-PCR analysis of the gene expression of (A) PAR-1, (B) S1PR1, (C) SphK1, & (D) SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for 0 h, 1 h, or 6 h. Western blot detection & quantification of the protein expression of PAR-1, S1PR1, SphK1, & SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for (E) 0 h, (F) 1 h, or (G) 6 h. The data are presented as the mean ± SD (n = 3), *p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32694981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-11424_rabbit-polyclonal-s1p1-edg-1-antibody-310202415175259.jpg)
![Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Expression of sphingosine-1-phosphate (S1P), interleukin (IL)-1 beta , & protease-activated receptor-1 (PAR-1) in isolated astrocytes. (A) Isolated & purified astrocytes were identified by bright-field microscopy & immunofluorescence for glial fibrillary acid protein (GFAP; red). Nuclei (DAPI) were labeled in blue. Scale bar = 100 μm. (B) Western blot & quantitative reverse transcription polymerase chain reaction (qRT-PCR) of the protein & mRNA expression, respectively, of S1P 0, 1, & 6 h after astrocytes were treated with lipopolysaccharide (LPS) or thrombin & Dabigatran (Dab) or PAR-1-inh (LPS only). (D) qRT-PCR & (E) enzyme-linked immunosorbent assay (ELISA) of the mRNA expression & secretion, respectively, of IL-1 beta 0, 1, & 6 h after astrocytes were treated with LPS or thrombin & Dab or PAR-1-inh (LPS only). (F) Immunofluorescence of astrocytes & quantification of relative mean integrated optical density (IOD; green fluorescence). PAR-1 was stained in green & nuclei (DAPI) were stained in blue. Scale bar = 100 μm. For (B–F), data are presented as the mean ± SD (n = 3). *p < 0.05; #p < 0.05 vs. the same group at 0 h; &p < 0.05 vs. the same group at 1 h. ns, not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32694981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-11424_rabbit-polyclonal-s1p1-edg-1-antibody-310202416205112.jpg)
![Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Western-Blot-NBP2-67129-img0011.jpg "Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0009.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse heart tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0008.jpg)
![Flow Cytometry: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of Jurkat cells with EDG1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Flow-Cytometry-NBP2-67129-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0003.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0004.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human kidney tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0005.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human liver tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0006.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse brain tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0007.jpg)
![Western blot analysis on different lysates with NBP2-67129 at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: PC-12 cell lysate Lane 3: Rat brain tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 20/40 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 60 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67129 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67129_-western-blot-639204598087360521.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody [NBP2-27291] - Analysis of S1P1/EDG-1 in FFPE human brain tissue using an isotype control (top left) and this antibody (bottom left, right)](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27291-img0002.jpg "Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody [NBP2-27291] - Analysis of S1P1/EDG-1 in FFPE human brain tissue using an isotype control (top left) and this antibody (bottom left, right)")
![Western Blot: S1P1/EDG-1 Antibody (9R8N3) [NBP3-16311] - Western blot analysis of extracts of various cell lines, using S1P1/EDG-1 Rabbit mAb (NBP3-16311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-9R8N3-Western-Blot-NBP3-16311-img0001.jpg "Western Blot: S1P1/EDG-1 Antibody (9R8N3) [NBP3-16311] - Western blot analysis of extracts of various cell lines, using S1P1/EDG-1 Rabbit mAb (NBP3-16311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NBP2-58024] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58024-img0001.jpg "Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NBP2-58024] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.")
![Western Blot: S1P1/EDG-1 Antibody (4F2) [NBP3-15216] - Positive WB detected in: SH-SY5Y whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U251 whole cell lysate. All lanes: S1P1/EDG-1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43 KDa. Observed band size: 43 KDa.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-4F2-Western-Blot-NBP3-15216-img0001.jpg "Western Blot: S1P1/EDG-1 Antibody (4F2) [NBP3-15216] - Positive WB detected in: SH-SY5Y whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U251 whole cell lysate. All lanes: S1P1/EDG-1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43 KDa. Observed band size: 43 KDa.")
