S100A4 Antibody (S100A4/2750R) - Azide and BSA Free

Western Blot: S100A4 Antibody (S100A4/2750R) - Azide and BSA Free [NBP3-08460] - Western Blot Analysis of human HeLa cell lysate using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R).

Immunocytochemistry/Immunofluorescence: S100A4 Antibody (S100A4/2750R) - Azide and BSA Free [NBP3-08460] - Immunofluorescence staining of A549 cells using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R) followed by goat anti- Mouse IgG-CF488 (green). Nuclei are stained with RedDot.

Immunohistochemistry-Paraffin: S100A4 Antibody (S100A4/2750R) - Azide and BSA Free [NBP3-08460] - Formalin-fixed, paraffin-embedded human Placenta stained with S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R).

Flow Cytometry: S100A4 Antibody (S100A4/2750R) - Azide and BSA Free [NBP3-08460] - Flow Cytometric Analysis of A549 cells using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: S100A4/2750R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

S100A4 Antibody (S100A4/2750R) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07587).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human S100A4 protein (around aa1-200) (exact sequence is proprietary) (Uniprot: P26447)
• Localization: Cytoplasmic and Nuclear
• Marker: Marker of Tumor Metastasis
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: S100A4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for S100A4 Antibody (S100A4/2750R) - Azide and BSA Free

• 18A2
• 42A
• Calvasculin
• CAPL
• CAPLS100 calcium binding protein A4 (calcium protein, calvasculin, metastasin
• fibroblast-specific protein-1,42A
• FSP1
• leukemia multidrug resistance associated protein
• malignant transformation suppression 1
• Metastasin
• MTS1
• murine placental homolog)
• P9KA
• PEL98
• Placental calcium-binding protein
• Protein Mts1
• protein S100-A4
• S100 calcium binding protein A4
• S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
• S100 calcium-binding protein A4
• S100A4

Background

S100A4 is encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S100A4