S100A4 Antibody (1F11)

Images

 
Genetic Strategies: Knockout Validated: S100A4 Antibody (1F11) [NBP2-36430] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and S100A4 knockout (KO) HeLa cell line. ...read more
Western Blot: S100A4 Antibody (1F11) [NBP2-36430] - Western blot analysis of S100A4 (1F11) in A375 and A549 cell lysate.
Immunocytochemistry/ Immunofluorescence: S100A4 Antibody (1F11) [NBP2-36430] - S100A4 (1F11) antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: S100A4 Antibody (1F11) [NBP2-36430] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal lung with mouse monoclonal S100A4 antibody (clone 1F11) at 5 ug/ml ...read more
Immunohistochemistry-Paraffin: S100A4 Antibody (1F11) [NBP2-36430] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human spleen with mouse monoclonal S100A4 antibody (clone 1F11) at 5 ug/ml ...read more
Immunohistochemistry-Paraffin: S100A4 Antibody (1F11) [NBP2-36430] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal skin with mouse monoclonal S100A4 antibody (clone 1F11) at 5 ug/ml ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clone
1F11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

S100A4 Antibody (1F11) Summary

Immunogen
Partial recombinant human S100A4 protein (between amino acids 1-200) [UniProt P26447]
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
S100A4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Knockout Validated
Positive Control
S100A4 Lysate (NBP2-64866)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for S100A4 Antibody (1F11)

  • 18A2
  • 42A
  • Calvasculin
  • CAPL
  • CAPLS100 calcium binding protein A4 (calcium protein, calvasculin, metastasin
  • fibroblast-specific protein-1,42A
  • FSP1
  • leukemia multidrug resistance associated protein
  • malignant transformation suppression 1
  • Metastasin
  • MTS1
  • murine placental homolog)
  • P9KA
  • PEL98
  • Placental calcium-binding protein
  • Protein Mts1
  • protein S100-A4
  • S100 calcium binding protein A4
  • S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
  • S100 calcium-binding protein A4
  • S100A4

Background

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium binding domains. This protein is ubiquitously overexpressed and is localized in the cytoplasm and/or nucleus. S100A4 may play a role in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of the S100A4 have been implicated in tumor metastasis. Recombinant mouse S100A4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for S100A4 Antibody (NBP2-36430) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

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Bioinformatics

Gene Symbol S100A4
Entrez
Uniprot